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PMID: 18786396 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Structure of the PduU shell protein from the Pdu microcompartment of Salmonella.

Structure (London, England : 1993) ·Vol. 16 ·No. 9 ·2008-09-10 ·Pages 1324-32

Crowley CS, Sawaya MR, Bobik TA, Yeates TO

Abstract

The Pdu microcompartment is a proteinaceous, subcellular structure that serves as an organelle for the metabolism of 1,2-propanediol in Salmonella enterica. It encapsulates several related enzymes within a shell composed of a few thousand protein subunits. Recent structural studies on the carboxysome, a related microcompartment involved in CO(2) fixation, have concluded that the major shell proteins from that microcompartment form hexamers that pack into layers comprising the facets of the shell. Here we report the crystal structure of PduU, a protein from the Pdu microcompartment, representing the first structure of a shell protein from a noncarboxysome microcompartment. Though PduU is a hexamer like other characterized shell proteins, it has undergone a circular permutation leading to dramatic differences in the hexamer pore. In view of the hypothesis that microcompartment metabolites diffuse across the outer shell through these pores, the unique structure of PduU suggests the possibility of a special functional role.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/chemistry,metabolism,physiology Cell Compartmentation/physiology Cell Wall/chemistry,ultrastructure Crystallography, X-Ray Models, Biological Models, Molecular Molecular Sequence Data Porins/chemistry,metabolism,physiology Protein Folding Protein Structure, Quaternary Salmonella enterica/chemistry,metabolism,ultrastructure Sequence Homology, Amino Acid
Chemicals
Bacterial Proteins Porins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Crowley Christopher S
University of California Los Angeles Molecular Biology Institute, University of California Los Angeles, Los Angeles, CA 90095, USA.
Sawaya Michael R
Bobik Thomas A
Yeates Todd O
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Article Info
Journal
Structure (London, England : 1993)
Abbr.
Structure
ISSN
0969-2126
DOI
10.1016/j.str.2008.05.013
Published
2008-09-10
Pages
1324-32
Language
English
Region
United States
NLM ID
101087697
PMCID
PMC5878062
Subset
IM
Grants
NIGMS NIH HHS · P01 GM031299 · United States
NIGMS NIH HHS · P01 GM031299-23 · United States
External Links
PubMed source DOI: 10.1016/j.str.2008.05.013 PMC Full Text View journal details
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