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PMID: 18765789 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Regulation of muscle development by DPF3, a novel histone acetylation and methylation reader of the BAF chromatin remodeling complex.

Genes & development ·Vol. 22 ·No. 17 ·2008-09-01 ·Pages 2370-84

Lange M, Kaynak B, Forster UB, Tönjes M, Fischer JJ, Grimm C, Schlesinger J, Just S, Dunkel I, Krueger T, Mebus S, Lehrach H, Lurz R, Gobom J, Rottbauer W, Abdelilah-Seyfried S, Sperling S

Abstract

Chromatin remodeling and histone modifications facilitate access of transcription factors to DNA by promoting the unwinding and destabilization of histone-DNA interactions. We present DPF3, a new epigenetic key factor for heart and muscle development characterized by a double PHD finger. DPF3 is associated with the BAF chromatin remodeling complex and binds methylated and acetylated lysine residues of histone 3 and 4. Thus, DPF3 may represent the first plant homeodomains that bind acetylated lysines, a feature previously only shown for the bromodomain. During development Dpf3 is expressed in the heart and somites of mouse, chicken, and zebrafish. Morpholino knockdown of dpf3 in zebrafish leads to incomplete cardiac looping and severely reduced ventricular contractility, with disassembled muscular fibers caused by transcriptional deregulation of structural and regulatory proteins. Promoter analysis identified Dpf3 as a novel downstream target of Mef2a. Taken together, DPF3 adds a further layer of complexity to the BAF complex by representing a tissue-specific anchor between histone acetylations as well as methylations and chromatin remodeling. Furthermore, this shows that plant homeodomain proteins play a yet unexplored role in recruiting chromatin remodeling complexes to acetylated histones.

MeSH Terms
Acetylation Amino Acid Sequence Animals Chick Embryo Chromatin Assembly and Disassembly DNA-Binding Proteins/biosynthesis,genetics Epigenesis, Genetic Heart/embryology Histones/metabolism Humans Methylation Mice Molecular Sequence Data Muscle Development/physiology Myocardium/metabolism Transcription Factors/biosynthesis,genetics Zebrafish/embryology,metabolism
Chemicals
DNA-Binding Proteins DPF3 protein, human Dpf3 protein, mouse Histones Transcription Factors
Authors & Affiliations
17 authors, click to expand affiliations / ORCID
Lange Martin
Group Cardiovascular Genetics, Department Vertebrate Genomics, Max Planck Institute for Molecular Genetics, Berlin 14195, Germany.
Kaynak Bogac
Forster Ulrike B
Tönjes Martje
Fischer Jenny J
Grimm Christina
Schlesinger Jenny
Just Steffen
Dunkel Ilona
Krueger Tammo
Mebus Siegrun
Lehrach Hans
Lurz Rudi
Gobom Johan
Rottbauer Wolfgang
Abdelilah-Seyfried Salim
Sperling Silke
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.471408
Published
2008-09-01
Pages
2370-84
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC2532927
Subset
IM
External Links
PubMed source DOI: 10.1101/gad.471408 PMC Full Text View journal details
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