Home LiteratureArticle Details
PMID: 18754598 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Self-assembly of brome mosaic virus capsids: insights from shorter time-scale experiments.

The journal of physical chemistry. A ·Vol. 112 ·No. 39 ·2008-10-02 ·Pages 9405-12

Chen C, Kao CC, Dragnea B

Abstract

An amended kinetic model for the self-assembly of empty capsids of brome mosaic virus is proposed. The model has been modified to account for a new feature in the assembly kinetics revealed by time-course light scattering experiments at higher temporal resolution than previously attempted. To be able to simulate the sharp takeoff from the initial lag phase to the growth phase in the kinetic curves, a monomer activation step was proposed.

MeSH Terms
Bromovirus/metabolism,physiology Capsid/metabolism,physiology Capsid Proteins/metabolism Dimerization Kinetics Models, Biological Protein Binding Scattering, Radiation Time Factors Virus Assembly
Chemicals
Capsid Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Chen Chao
Department of Chemistry, Indiana University, Bloomington, Indiana 47405, USA. chachen@indiana.edu
Kao C Cheng
Dragnea Bogdan
References (34)
34 references, click to expand
  1. Effects of the cowpea chlorotic mottle bromovirus beta-hexamer structure on virion assembly.
    Virology. 2003 Feb 15;306(2):280-8 PMID: 12642101
  2. A theoretical model successfully identifies features of hepatitis B virus capsid assembly.
    Biochemistry. 1999 Nov 2;38(44):14644-52 PMID: 10545189
  3. Potential subversion of autophagosomal pathway by picornaviruses.
    Autophagy. 2008 Apr;4(3):286-9 PMID: 18094610
  4. Calculation of the free energy of association for protein complexes.
    Protein Sci. 1992 Jan;1(1):169-81 PMID: 1339024
  5. Competing hydrophobic and screened-coulomb interactions in hepatitis B virus capsid assembly.
    Biophys J. 2004 Jun;86(6):3905-13 PMID: 15189887
  6. Scaffolding protein regulates the polymerization of P22 coat subunits into icosahedral shells in vitro.
    J Mol Biol. 1988 Aug 20;202(4):743-57 PMID: 3262767
  7. Nucleation and growth phases in the polymerization of coat and scaffolding subunits into icosahedral procapsid shells.
    Biophys J. 1993 Mar;64(3):824-35 PMID: 8471727
  8. The crystallographic structure of brome mosaic virus.
    J Mol Biol. 2002 Mar 15;317(1):95-108 PMID: 11916381
  9. Self-assembly of brome mosaic virus capsids. Kinetic study using neutron and X-ray solution scattering.
    J Mol Biol. 1983 Mar 15;164(4):645-50 PMID: 6842602
  10. Assembly in vitro of bacteriophage P22 procapsids from purified coat and scaffolding subunits.
    J Mol Biol. 1982 Apr 15;156(3):633-65 PMID: 6750133
  11. RNA viruses: stabilization of brome mosaic virus.
    Philos Trans R Soc Lond B Biol Sci. 1976 Nov 30;276(943):99-107 PMID: 13438
  12. Weak protein-protein interactions are sufficient to drive assembly of hepatitis B virus capsids.
    Biochemistry. 2002 Oct 1;41(39):11525-31 PMID: 12269796
  13. Aggregation states of brome mosaic virus protein.
    Virology. 1974 Sep;61(1):160-7 PMID: 4411901
  14. Theoretical aspects of virus capsid assembly.
    J Mol Recognit. 2005 Nov-Dec;18(6):479-90 PMID: 16193532
  15. Structure of small virus-like particles assembled from the L1 protein of human papillomavirus 16.
    Mol Cell. 2000 Mar;5(3):557-67 PMID: 10882140
  16. Local rule-based theory of virus shell assembly.
    Proc Natl Acad Sci U S A. 1994 Aug 2;91(16):7732-6 PMID: 8052652
  17. Hydrophobic interactions.
    Angew Chem Int Ed Engl. 1967 Mar;6(3):195-206 PMID: 4962948
  18. Energetics of quasiequivalence: computational analysis of protein-protein interactions in icosahedral viruses.
    Biophys J. 1998 Jan;74(1):546-58 PMID: 9449355
  19. Factors affecting the assembly of some spherical viruses.
    Virology. 1969 Oct;39(2):296-311 PMID: 5344288
  20. Distinguishing reversible from irreversible virus capsid assembly.
    J Mol Biol. 2007 Feb 9;366(1):14-8 PMID: 17157314
  21. Evolutionary conservation in the hepatitis B virus core structure: comparison of human and duck cores.
    Structure. 1995 Oct 15;3(10):1009-19 PMID: 8589996
  22. Self-assembly of brome mosaic virus protein into capsids. Initial and final states of aggregation.
    J Mol Biol. 1983 Mar 15;164(4):589-603 PMID: 6842600
  23. Are weak protein-protein interactions the general rule in capsid assembly?
    Virology. 2003 Oct 25;315(2):269-74 PMID: 14585329
  24. Phage P22 procapsids equilibrate with free coat protein subunits.
    J Mol Biol. 2007 Jan 12;365(2):513-22 PMID: 17067636
  25. Mechanism of capsid assembly for an icosahedral plant virus.
    Virology. 2000 Nov 25;277(2):450-6 PMID: 11080492
  26. Local rules simulation of the kinetics of virus capsid self-assembly.
    Biophys J. 1998 Dec;75(6):2626-36 PMID: 9826587
  27. To build a virus capsid. An equilibrium model of the self assembly of polyhedral protein complexes.
    J Mol Biol. 1994 Aug 5;241(1):59-67 PMID: 8051707
  28. Deciphering the kinetic mechanism of spontaneous self-assembly of icosahedral capsids.
    Nano Lett. 2007 Feb;7(2):338-44 PMID: 17297998
  29. Three-dimensional analysis of a viral RNA replication complex reveals a virus-induced mini-organelle.
    PLoS Biol. 2007 Sep;5(9):e220 PMID: 17696647
  30. In vitro papillomavirus capsid assembly analyzed by light scattering.
    Virology. 2004 Aug 1;325(2):320-7 PMID: 15246271
  31. Self-assembly of polyhedral shells: a molecular dynamics study.
    Phys Rev E Stat Nonlin Soft Matter Phys. 2004 Nov;70(5 Pt 1):051905 PMID: 15600654
  32. Classical nucleation theory of virus capsids.
    Biophys J. 2006 Mar 15;90(6):1939-48 PMID: 16387781
  33. The self-assembly of a nucleic-acid free pseudo-top component for a small spherical virus.
    Virology. 1968 Sep;36(1):146-9 PMID: 4970235
  34. Observed hysteresis of virus capsid disassembly is implicit in kinetic models of assembly.
    J Biol Chem. 2003 May 16;278(20):18249-55 PMID: 12639968
Article Info
Journal
The journal of physical chemistry. A
Abbr.
J Phys Chem A
ISSN
1520-5215
DOI
10.1021/jp802498z
Email
chachen@indiana.edu
Published
2008-10-02
Epub
2008-00-28
Pages
9405-12
Language
English
Region
United States
NLM ID
9890903
PMCID
PMC2753409
Subset
IM
Grants
NIGMS NIH HHS · R01 GM081029 · United States
NIGMS NIH HHS · R01 GM081029-01 · United States
External Links
PubMed source DOI: 10.1021/jp802498z PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2