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PMID: 18701071 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Mouse cones require an arrestin for normal inactivation of phototransduction.

Neuron ·Vol. 59 ·No. 3 ·2008-08-14 ·Pages 462-74

Nikonov SS, Brown BM, Davis JA, Zuniga FI, Bragin A, Pugh EN, Craft CM

Abstract

Arrestins are proteins that arrest the activity of G protein-coupled receptors (GPCRs). While it is well established that normal inactivation of photoexcited rhodopsin, the GPCR of rod phototransduction, requires arrestin (Arr1), it has been controversial whether the same requirement holds for cone opsin inactivation. Mouse cone photoreceptors express two distinct visual arrestins: Arr1 and Arr4. By means of recordings from cones of mice with one or both arrestins knocked out, this investigation establishes that a visual arrestin is required for normal cone inactivation. Arrestin-independent inactivation is 70-fold more rapid in cones than in rods, however. Dual arrestin expression in cones could be a holdover from ancient genome duplication events that led to multiple isoforms of arrestin, allowing evolutionary specialization of one form while the other maintains the basic function.

MeSH Terms
Analysis of Variance Animals Arrestin/classification,deficiency,metabolism Electrophysiology Light Membrane Potentials/genetics,radiation effects Mice Mice, Inbred C57BL Mice, Knockout Microscopy, Confocal Reaction Time/physiology Retina/cytology Retinal Cone Photoreceptor Cells/physiology Rod Opsins/pharmacology Vision, Ocular/physiology,radiation effects
Chemicals
Arrestin Rod Opsins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Nikonov Sergei S
F.M. Kirby Center for Molecular Ophthalmology, Department of Ophthalmology, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104-6069, USA.
Brown Bruce M
Davis Jason A
Zuniga Freddi I
Bragin Alvina
Pugh Edward N
Craft Cheryl M
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Article Info
Journal
Neuron
Abbr.
Neuron
ISSN
1097-4199
DOI
10.1016/j.neuron.2008.06.011
Published
2008-08-14
Pages
462-74
Language
English
Region
United States
NLM ID
8809320
PMCID
PMC2610856
Subset
IM
Grants
NEI NIH HHS · EY016453 · United States
NEI NIH HHS · R01 EY002660-30 · United States
NEI NIH HHS · R01 EY015851-02 · United States
NEI NIH HHS · R01 EY015851-04 · United States
NEI NIH HHS · EY02660 · United States
NIGMS NIH HHS · GM799102 · United States
NEI NIH HHS · R01 EY016453 · United States
NIGMS NIH HHS · F31 GM079910 · United States
NEI NIH HHS · R01 EY015851-03 · United States
NEI NIH HHS · EY015851 · United States
NEI NIH HHS · R01 EY015851-01A2 · United States
NEI NIH HHS · R01 EY002660 · United States
NEI NIH HHS · EY03040 · United States
External Links
PubMed source DOI: 10.1016/j.neuron.2008.06.011 PMC Full Text View journal details
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