Eleven golden rules of quantitative RT-PCR.

The Plant cell ·Vol. 20 ·No. 7 ·2008-07-00 ·Pages 1736-7

Udvardi MK, Czechowski T, Scheible WR

Abstract

暂无摘要

MeSH Terms

Reproducibility of Results Reverse Transcriptase Polymerase Chain Reaction/methods,standards

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Udvardi Michael K

Czechowski Tomasz

Scheible Wolf-Rüdiger

References (6)

6 references, click to expand

Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes.
Genome Biol. 2002 Jun 18;3(7):RESEARCH0034 PMID: 12184808
Properties of the reverse transcription reaction in mRNA quantification.
Clin Chem. 2004 Mar;50(3):509-15 PMID: 14726469
Real-time RT-PCR profiling of over 1400 Arabidopsis transcription factors: unprecedented sensitivity reveals novel root- and shoot-specific genes.
Plant J. 2004 Apr;38(2):366-79 PMID: 15078338
Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper--Excel-based tool using pair-wise correlations.
Biotechnol Lett. 2004 Mar;26(6):509-15 PMID: 15127793
Genome-wide identification and testing of superior reference genes for transcript normalization in Arabidopsis.
Plant Physiol. 2005 Sep;139(1):5-17 PMID: 16166256
Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data.
Neurosci Lett. 2003 Mar 13;339(1):62-6 PMID: 12618301

Article Info

Journal

The Plant cell

Abbr.

Plant Cell

ISSN

1040-4651

DOI

Published

2008-07-00

Epub

2008-00-29

Pages

1736-7

Language

English

Region

England

NLM ID

9208688

PMCID

PMC2518243

Subset

Corrections

CommentIn

PMID: 18664610

External Links

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