Abstract
MicroRNAs (miRNA/miR) are a class of small noncoding RNAs implicated in the pathogenesis of various malignancies. In the current study, using micro(RNA) arrays, we found a ubiquitous loss of miR-126 expression in colon cancer lines when compared to normal human colon epithelia. Reconstitution of miR-126 in colon cancer cells resulted in a significant growth reduction as evidenced in clonogenic assays. A search for miR-126 gene targets revealed p85beta, a regulatory subunit involved in stabilizing and propagating the phosphatidylinositol 3-kinase (PI3K) signal, as one of the potential substrates. Restoration of miR-126 in cancer cells induced a > or =3-fold reduction in p85beta protein levels, with no concomitant change in p85alpha, a gene that is functionally related to p85beta but not a supposed target of miR-126. Additionally, using reporter constructs, we show that the p85beta-3' untranslated region is directly targeted by miR-126. Furthermore, this miR-126 mediated reduction of p85beta was accompanied by a substantial reduction in phosphorylated AKT levels in the cancer cells, suggesting an impairment in PI3K signaling. Finally, in a panel of matched normal colon and primary colon tumors, each of the tumors demonstrated miR-126 down-regulation together with an increase in the p85beta protein level. Taken together, we propose that miR-126 regulates PI3K signaling partly by targeting p85beta, and that the loss of miR-126 may provide a selective growth advantage during colon carcinogenesis.
MeSH Terms
Cell Line, Tumor
Cell Transformation, Neoplastic/genetics,metabolism
Colonic Neoplasms/enzymology,genetics,metabolism
Down-Regulation
Humans
MicroRNAs/genetics,metabolism
Phosphatidylinositol 3-Kinases/genetics,metabolism
Phosphoinositide-3 Kinase Inhibitors
Protein Subunits/genetics,metabolism
Signal Transduction
Chemicals
MIRN126 microRNA, human
MicroRNAs
Phosphoinositide-3 Kinase Inhibitors
Protein Subunits
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Guo Chunguang
Department of Medicine and Ireland Cancer Center, Case Medical Center and Case Western Reserve University, Cleveland, OH 44106, USA.
Sah Jerome F
Beard Lydia
Willson James K V
Markowitz Sanford D
Guda Kishore
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