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PMID: 18662310 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Effects of transgenic fructan-producing potatoes on the community structure of rhizosphere and phyllosphere bacteria.

FEMS microbiology ecology ·Vol. 66 ·No. 2 ·2008-11-00 ·Pages 411-25

Becker R, Behrendt U, Hommel B, Kropf S, Ulrich A

Abstract

The rhizosphere and phyllosphere microbial communities of transgenic potatoes producing fructan were studied in comparison with isogenic controls and conventional varieties in a field release experiment over a period of 3 years. Population densities and 16S rRNA gene-based terminal restriction fragment length polymorphism (T-RFLP) analysis of the rhizosphere bacterial community only displayed the influence of annual and seasonal effects and the influence of field heterogeneity. In contrast, the T-RFLP analysis of the phyllosphere bacteria revealed in two of the 3 years significant differences in the community structure between the transgenic lines producing inulin and the other variants. This effect was studied in more detail through the analysis of bacterial isolates and a 16S rRNA gene clone library obtained from a transgenic line and the control. Both methods revealed a lower genetic diversity in the transgenic line and changes in the abundance of several bacterial groups. The isolates of the transgenic line were dominated by Bacilli, whereas most of the control isolates represented Actinobacteria. The clones were dominated by Proteobacteria, with main differences between both variants in Deltaproteobacteria, Bacilli and Bacteroidetes. However, all in all, the impact of the transgenic lines did not exceed the natural variability of the phyllosphere community structure on potato plants.

MeSH Terms

Bacteria/classification,genetics,growth & development,isolation & purification Ecosystem Fructans/metabolism Molecular Sequence Data Plant Roots/microbiology Plants, Genetically Modified/microbiology Polymorphism, Restriction Fragment Length RNA, Ribosomal, 16S/genetics Seasons Sequence Analysis, DNA Soil Microbiology Solanum tuberosum/genetics,metabolism,microbiology

Chemicals

Fructans RNA, Ribosomal, 16S

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Becker Regina

Leibniz-Zentrum für Agrarlandschaftsforschung (ZALF), Institut für Landschaftsstoffdynamik, Müncheberg, Germany.

Behrendt Undine

Hommel Bernd

Kropf Siegfried

Ulrich Andreas

Article Info

Journal

FEMS microbiology ecology

Abbr.

FEMS Microbiol Ecol

ISSN

0168-6496

DOI

10.1111/j.1574-6941.2008.00562.x

Published

2008-11-00

Epub

2008-00-24

Pages

411-25

Language

English

Region

England

NLM ID

8901229

Subset

Databases

GENBANK

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PubMed source DOI: 10.1111/j.1574-6941.2008.00562.x View journal details

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Effects of transgenic fructan-producing potatoes on the community structure of rhizosphere and phyllosphere bacteria.

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