Non-isotopic detection of chromosome 1 in human meiosis and demonstration of disomic sperm nuclei.
Nonradioactive in situ hybridization with the biotin-labeled chromosome 1-specific probe pUC1.77 was performed on human mitotic and meiotic chromosomes, and on sperm nuclei. The streptavidine-horseradish-peroxidase and diaminobenzidine detection system demonstrated heteromorphisms in the 1q12 heterochromatic region, not only in mitotic cells but also in mature sperm heads. The localization of chromosome 1 could be traced through all meiotic stages and in the sperm nuclei. The frequency of chromosome 1 disomy in human sperm, as indicated by two distinct hybridization signals, was calculated to be 0.41%.
19 references, click to expand
-
Trisomy 1 in an eight cell human pre-embryo.J Med Genet. 1987 Jan;24(1):60-4 PMID: 3806641
-
Direct chromosomal analysis of human spermatozoa: preliminary results from 18 normal men.Am J Hum Genet. 1982 May;34(3):459-68 PMID: 7081223
-
Cloning of human satellite III DNA: different components are on different chromosomes.Nucleic Acids Res. 1979 Jul 25;6(10):3177-97 PMID: 573470
-
The frequency of aneuploidy among individual chromosomes in 6,821 human sperm chromosome complements.Cytogenet Cell Genet. 1990;53(2-3):103-7 PMID: 2369835
-
The Y chromosome in human spermatozoa.Nature. 1970 Jun 6;226(5249):961-2 PMID: 4192297
-
Detection of chromosome aberrations in interphase tumor nuclei by nonradioactive in situ hybridization.Cancer Genet Cytogenet. 1989 Oct 1;42(1):87-98 PMID: 2551485
-
Estimation of aneuploidy levels in human spermatozoa using chromosome specific probes and in situ hybridisation.Hum Genet. 1984;66(2-3):234-8 PMID: 6201432
-
Rapid interphase and metaphase assessment of specific chromosomal changes in neuroectodermal tumor cells by in situ hybridization with chemically modified DNA probes.Exp Cell Res. 1988 Jun;176(2):199-220 PMID: 3288483
-
Determination of Y chromosome aneuploidy in human sperm nuclei by nonradioactive in situ hybridization.Am J Hum Genet. 1990 Mar;46(3):553-8 PMID: 2309704
-
Somatic pairing of chromosome 1 centromeres in interphase nuclei of human cerebellum.Hum Genet. 1989 Oct;83(3):231-4 PMID: 2793166
-
Staining of some specific regions of human chromosomes, particularly the secondary constriction of No. 9.Nat New Biol. 1972 Jul 26;238(82):122-4 PMID: 4114702
-
The chromosome constitution of 1000 human spermatozoa.Hum Genet. 1983;63(4):305-9 PMID: 6683243
-
Detection of chromosome aneuploidy in interphase nuclei from human primary breast tumors using chromosome-specific repetitive DNA probes.Cancer Res. 1988 Oct 15;48(20):5825-30 PMID: 3167839
-
Specific staining of the human No. 1 chromosome in spermatozoa.Humangenetik. 1973;20(2):171-3 PMID: 4131814
-
Human gametes and zygotes studied by nonradioactive in situ hybridization.Cytogenet Cell Genet. 1990;53(1):15-9 PMID: 2182292
-
Technique for identifying Y chromosomes in human interphase nuclei.Nature. 1970 Apr 4;226(5240):78-80 PMID: 4190810
-
Chromosomal abnormalities in human sperm: comparisons among four healthy men.Hum Genet. 1984;66(2-3):193-201 PMID: 6714980
-
Variation in the frequency and type of sperm chromosomal abnormalities among normal men.Hum Genet. 1987 Oct;77(2):108-14 PMID: 3653884
-
Spatial distribution of chromosomes 1 and Y in human spermatozoa.J Reprod Fertil. 1975 Dec;45(3):515-7 PMID: 1206650
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: product@genelibs.com
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
