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PMID: 18628952 Published · epublish English Journal Article Research Support, Non-U.S. Gov't

Protein solubility and folding enhancement by interaction with RNA.

PloS one ·Vol. 3 ·No. 7 ·2008-07-16 ·Pages e2677

Choi SI, Han KS, Kim CW, Ryu KS, Kim BH, Kim KH, Kim SI, Kang TH, Shin HC, Lim KH, Kim HK, Hyun JM, Seong BL

Abstract

While basic mechanisms of several major molecular chaperones are well understood, this machinery has been known to be involved in folding of only limited number of proteins inside the cells. Here, we report a chaperone type of protein folding facilitated by interaction with RNA. When an RNA-binding module is placed at the N-terminus of aggregation-prone target proteins, this module, upon binding with RNA, further promotes the solubility of passenger proteins, potentially leading to enhancement of proper protein folding. Studies on in vitro refolding in the presence of RNA, coexpression of RNA molecules in vivo and the mutants with impaired RNA binding ability suggests that RNA can exert chaperoning effect on their bound proteins. The results suggest that RNA binding could affect the overall kinetic network of protein folding pathway in favor of productive folding over off-pathway aggregation. In addition, the RNA binding-mediated solubility enhancement is extremely robust for increasing soluble yield of passenger proteins and could be usefully implemented for high-throughput protein expression for functional and structural genomic research initiatives. The RNA-mediated chaperone type presented here would give new insights into de novo folding in vivo.

MeSH Terms
Cytosol/metabolism DNA/chemistry Enhancer Elements, Genetic Humans Models, Genetic Molecular Chaperones/chemistry Mutagenesis, Site-Directed Nucleic Acid Conformation Protein Binding Protein Denaturation Protein Folding Protein Structure, Tertiary Proteins/chemistry RNA/chemistry Solubility
Chemicals
Molecular Chaperones Proteins RNA DNA
Authors & Affiliations
13 authors, click to expand affiliations / ORCID
Choi Seong Il
Institute of Life Science and Biotechnology, Yonsei University, Seodaemun-Gu, Seoul, Korea.
Han Kyoung Sim
Kim Chul Woo
Ryu Ki-Sun
Kim Byung Hee
Kim Kyun-Hwan
Kim Seo-Il
Kang Tae Hyun
Shin Hang-Cheol
Lim Keo-Heun
Kim Hyo Kyung
Hyun Jeong-Min
Seong Baik L
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Article Info
Journal
PloS one
Abbr.
PLoS One
ISSN
1932-6203
Published
2008-07-16
Epub
2008-00-16
Pages
e2677
Language
English
Region
United States
NLM ID
101285081
PMCID
PMC2444022
Subset
IM
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