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PMID: 18621711 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Evolution of an RNP assembly system: a minimal SMN complex facilitates formation of UsnRNPs in Drosophila melanogaster.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 105 ·No. 29 ·2008-07-22 ·Pages 10045-50

Kroiss M, Schultz J, Wiesner J, Chari A, Sickmann A, Fischer U

Abstract

In vertebrates, assembly of spliceosomal uridine-rich small nuclear ribonucleoproteins (UsnRNPs) is mediated by the SMN complex, a macromolecular entity composed of the proteins SMN and Gemins 2-8. Here we have studied the evolution of this machinery using complete genome assemblies of multiple model organisms. The SMN complex has gained complexity in evolution by a blockwise addition of Gemins onto an ancestral core complex composed of SMN and Gemin2. In contrast to this overall evolutionary trend to more complexity in metazoans, orthologs of most Gemins are missing in dipterans. In accordance with these bioinformatic data a previously undescribed biochemical purification strategy elucidated that the dipteran Drosophila melanogaster contains an SMN complex of remarkable simplicity. Surprisingly, this minimal complex not only mediates the assembly reaction in a manner very similar to its vertebrate counterpart, but also prevents misassembly onto nontarget RNAs. Our data suggest that only a minority of Gemins are required for the assembly reaction per se, whereas others may serve additional functions in the context of UsnRNP biogenesis. The evolution of the SMN complex is an interesting example of how the simplification of a biochemical process contributes to genome compaction.

MeSH Terms
Animals Cell Cycle Proteins/chemistry,genetics,metabolism Cell Line Cyclic AMP Response Element-Binding Protein/chemistry,genetics,metabolism Drosophila Proteins/biosynthesis,chemistry,genetics,metabolism Drosophila melanogaster/genetics,metabolism Evolution, Molecular Genome, Insect Histone Chaperones Intracellular Signaling Peptides and Proteins/chemistry,genetics,metabolism Models, Genetic Multiprotein Complexes Nerve Tissue Proteins/chemistry,genetics,metabolism RNA Splicing RNA-Binding Proteins/chemistry,genetics,metabolism Ribonucleoproteins, Small Nuclear/biosynthesis,chemistry,genetics SMN Complex Proteins Spliceosomes/metabolism Transcription Factors/chemistry,genetics,metabolism
Chemicals
Cell Cycle Proteins Cyclic AMP Response Element-Binding Protein Drosophila Proteins Hira protein, Drosophila Histone Chaperones Intracellular Signaling Peptides and Proteins Multiprotein Complexes Nerve Tissue Proteins RNA-Binding Proteins Ribonucleoproteins, Small Nuclear Rig protein, Drosophila SMN Complex Proteins Transcription Factors
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Kroiss Matthias
Department of Biochemistry, Theodor Boveri Institute, Biocenter, Am Hubland, 97074 Würzburg, Germany.
Schultz Jörg
Wiesner Julia
Chari Ashwin
Sickmann Albert
Fischer Utz
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
1091-6490
DOI
10.1073/pnas.0802287105
Published
2008-07-22
Epub
2008-00-10
Pages
10045-50
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC2481332
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.0802287105 PMC Full Text View journal details
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