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PMID: 1861981 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Distribution and characterization of helix-loop-helix enhancer-binding proteins from pancreatic beta cells and lymphocytes.

Nucleic acids research ·Vol. 19 ·No. 14 ·1991-07-25 ·Pages 3893-9

Aronheim A, Ohlsson H, Park CW, Edlund T, Walker MD

Abstract

Transcription of a number of mammalian genes is controlled in part by closely-related DNA elements sharing a CAxxTG consensus sequence (E boxes). In this report, we survey cell extracts from a variety of mammalian cell lineages for ability to bind to the E box denoted IEB1/kappa E1, which plays an important role in expression of both insulin and immunoglobulin kappa genes. Insulin enhancer factor 1 (IEF1), a binding activity previously identified in beta cells, was also present in pituitary endocrine cells but absent in 7 other mammalian cell lines tested. A distinct binding activity, lymphoid enhancer factor 1 (LEF1), was observed in several lymphoid cell lines, but was absent from all nonlymphoid cells tested. IEF1 and LEF1 were distinct according to electrophoretic mobility, and DNA binding specificity. As previously reported, both beta cell and lymphoid cell factors are recognized by antibodies to helix-loop-helix (HLH) proteins, indicating that they may contain functional helix-loop-helix dimerization domains. To directly demonstrate this, we showed that the binding factors are able to interact in vitro with the HLH domain of a characterized HLH protein. These results support the notion that HLH proteins play a key role in cell-specific transcriptional regulation in cells from endocrine and lymphocyte lineages.

MeSH Terms
Animals Antibodies/immunology Base Sequence Basic Helix-Loop-Helix Transcription Factors Cell Line DNA DNA-Binding Proteins/chemistry,immunology,metabolism Enhancer Elements, Genetic Homeodomain Proteins Islets of Langerhans/metabolism LIM-Homeodomain Proteins Lymphocytes/metabolism Lymphoid Enhancer-Binding Factor 1 Mice Molecular Sequence Data Nerve Tissue Proteins Nuclear Proteins/metabolism Organ Specificity Protein Conformation Rats Transcription Factors
Chemicals
Antibodies Basic Helix-Loop-Helix Transcription Factors DNA-Binding Proteins Homeodomain Proteins IEBP1 protein, rat LIM-Homeodomain Proteins Lef1 protein, mouse Lef1 protein, rat Lymphoid Enhancer-Binding Factor 1 Nerve Tissue Proteins Nuclear Proteins Transcription Factors insulin gene enhancer binding protein Isl-1 DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Aronheim A
Department of Biochemistry, Weizmann Institute of Science, Rehovot, Israel.
Ohlsson H
Park C W
Edlund T
Walker M D
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/19.14.3893
Published
1991-07-25
Pages
3893-9
Language
English
Region
England
NLM ID
0411011
PMCID
PMC328480
Subset
IM
Grants
NIGMS NIH HHS · GM38817 · United States
External Links
PubMed source DOI: 10.1093/nar/19.14.3893 PMC Full Text View journal details
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