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PMID: 18568776 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Expression of multidrug resistance genes in normal and cancer stem cells.

Cancer investigation ·Vol. 26 ·No. 5 ·2008-06-00 ·Pages 535-42

Shervington A, Lu C

Abstract

CD133 (stem marker) and drug resistant gene (mdr1 and bcl-2) expression were detected in human glioblastoma and normal brain tissues. Glioma tissues contained higher ratio of CD133+ cells compared to normal tissues ( approximately 10 fold increase). The chemosensitivity assay showed that GOS-3 and NHA CD133+ possess greater resistance towards drugs compared to CD133- fractions. This study revealed for the first time that: a) serum deprivation enriched CD133 expression and demonstrated a direct co-expression between CD133 and drug resistant in GOS-3 cells and b) higher expression of CD133 and drug resistance were found in glioblastoma tissues in comparison to normal brain tissues.

MeSH Terms
AC133 Antigen ATP Binding Cassette Transporter, Subfamily B ATP Binding Cassette Transporter, Subfamily B, Member 1/genetics,metabolism Antigens, CD/genetics,metabolism Antineoplastic Agents/pharmacology Brain Neoplasms/genetics,immunology,metabolism,pathology Cell Line, Tumor Cell Proliferation Cell Survival Culture Media, Serum-Free Dose-Response Relationship, Drug Drug Resistance, Multiple/genetics Drug Resistance, Neoplasm/genetics Flow Cytometry Gene Expression Profiling Gene Expression Regulation, Neoplastic Glioblastoma/genetics,immunology,metabolism,pathology Glycoproteins/genetics,metabolism Humans Immunomagnetic Separation Inhibitory Concentration 50 Intermediate Filament Proteins/metabolism Neoplastic Stem Cells/drug effects,immunology,metabolism Nerve Tissue Proteins/metabolism Nestin Peptides/genetics,metabolism Proto-Oncogene Proteins c-bcl-2/genetics,metabolism Reverse Transcriptase Polymerase Chain Reaction Up-Regulation
Chemicals
ABCB1 protein, human AC133 Antigen ATP Binding Cassette Transporter, Subfamily B ATP Binding Cassette Transporter, Subfamily B, Member 1 Antigens, CD Antineoplastic Agents Culture Media, Serum-Free Glycoproteins Intermediate Filament Proteins NES protein, human Nerve Tissue Proteins Nestin PROM1 protein, human Peptides Proto-Oncogene Proteins c-bcl-2
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Shervington A
Brain Tumor North West, Faculty of Science and Technology, University of Central Lancashire, Preston, United Kingdom. aashervington@uclan.ac.uk
Lu C
Article Info
Journal
Cancer investigation
Abbr.
Cancer Invest
ISSN
1532-4192
Published
2008-06-00
Pages
535-42
Language
English
Region
England
NLM ID
8307154
Subset
IM
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