Abstract
CD133 (stem marker) and drug resistant gene (mdr1 and bcl-2) expression were detected in human glioblastoma and normal brain tissues. Glioma tissues contained higher ratio of CD133+ cells compared to normal tissues ( approximately 10 fold increase). The chemosensitivity assay showed that GOS-3 and NHA CD133+ possess greater resistance towards drugs compared to CD133- fractions. This study revealed for the first time that: a) serum deprivation enriched CD133 expression and demonstrated a direct co-expression between CD133 and drug resistant in GOS-3 cells and b) higher expression of CD133 and drug resistance were found in glioblastoma tissues in comparison to normal brain tissues.
MeSH Terms
AC133 Antigen
ATP Binding Cassette Transporter, Subfamily B
ATP Binding Cassette Transporter, Subfamily B, Member 1/genetics,metabolism
Antigens, CD/genetics,metabolism
Antineoplastic Agents/pharmacology
Brain Neoplasms/genetics,immunology,metabolism,pathology
Cell Line, Tumor
Cell Proliferation
Cell Survival
Culture Media, Serum-Free
Dose-Response Relationship, Drug
Drug Resistance, Multiple/genetics
Drug Resistance, Neoplasm/genetics
Flow Cytometry
Gene Expression Profiling
Gene Expression Regulation, Neoplastic
Glioblastoma/genetics,immunology,metabolism,pathology
Glycoproteins/genetics,metabolism
Humans
Immunomagnetic Separation
Inhibitory Concentration 50
Intermediate Filament Proteins/metabolism
Neoplastic Stem Cells/drug effects,immunology,metabolism
Nerve Tissue Proteins/metabolism
Nestin
Peptides/genetics,metabolism
Proto-Oncogene Proteins c-bcl-2/genetics,metabolism
Reverse Transcriptase Polymerase Chain Reaction
Up-Regulation
Chemicals
ABCB1 protein, human
AC133 Antigen
ATP Binding Cassette Transporter, Subfamily B
ATP Binding Cassette Transporter, Subfamily B, Member 1
Antigens, CD
Antineoplastic Agents
Culture Media, Serum-Free
Glycoproteins
Intermediate Filament Proteins
NES protein, human
Nerve Tissue Proteins
Nestin
PROM1 protein, human
Peptides
Proto-Oncogene Proteins c-bcl-2
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Shervington A
Brain Tumor North West, Faculty of Science and Technology, University of Central Lancashire, Preston, United Kingdom. aashervington@uclan.ac.uk
Lu C