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PMID: 1856854 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Binding of the anticodon domain of tRNA(fMet) to Escherichia coli methionyl-tRNA synthetase.

Journal of molecular biology ·Vol. 220 ·No. 2 ·1991-07-20 ·Pages 205-8

Meinnel T, Mechulam Y, Blanquet S, Fayat G

Abstract

A stem and loop RNA domain carrying the methionine anticodon (CAU) was designed from the tRNA(fMet) sequence and produced in vitro. This domain makes a complex with methionyl-tRNA synthetase (Kd = 38(+/- 5) microM; 25 degrees C, pH 7.6, 7 mM-MgCl2). The formation of this complex is dependent on the presence of the cognate CAU anticodon sequence. Recognition of this RNA domain is abolished by a methionyl-tRNA synthetase mutation known to alter the binding of tRNA(Met).

MeSH Terms

Anticodon Base Sequence Calorimetry Escherichia coli/enzymology Kinetics Methionine-tRNA Ligase/metabolism Molecular Sequence Data Nucleic Acid Conformation Protein Binding RNA, Transfer, Amino Acyl/chemical synthesis,genetics,metabolism RNA, Transfer, Met

Chemicals

Anticodon RNA, Transfer, Amino Acyl RNA, Transfer, Met tRNA, formylmethionine- Methionine-tRNA Ligase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Meinnel T

Laboratoire de Biochimie, Unité Associée 240 du Centre National de la Recherche Scientifique Ecole Polytechnique, Palaiseau, France.

Mechulam Y

Blanquet S

Fayat G

Article Info

Journal

Journal of molecular biology

Abbr.

J Mol Biol

ISSN

0022-2836

DOI

10.1016/0022-2836(91)90003-o

Published

1991-07-20

Pages

205-8

Language

English

Region

England

NLM ID

2985088R

Subset

External Links

PubMed source DOI: 10.1016/0022-2836(91)90003-o View journal details

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Binding of the anticodon domain of tRNA(fMet) to Escherichia coli methionyl-tRNA synthetase.

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