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PMID: 185621 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Rous-sarcoma-virus-transformed fibroblasts having low levels of plasminogen activator.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 10 ·1976-10-00 ·Pages 3613-7

Wolf BA, Goldberg AR

Abstract

Several clones of transformed chick embryo fibroblasts infected with wild-type B77-C or Prague-C strain of Rous sarcoma virus have been isolated from soft agar suspension. These clones were screened for plasminogen activator activity by overlaying monolayer cultures with medium containing agar, casein, and chicken plasminogen. Twenty-three percent of all of the isolated clones showed little caseinolytic activity, 42% had intermediate activity, and 35% had high activity. Although the clones with low plasminogen activator activity had no more than twice the activity shown by uninfected fibroblasts, they did not differ significantly from clones possessing high levels of plasminogen activator in their morphology, 2-deoxyglucose transport, or efficiency of colony formation in soft-agar.

MeSH Terms
Avian Sarcoma Viruses Biological Transport Cell Division Cell Transformation, Neoplastic/pathology Clone Cells Deoxyglucose/metabolism Fibrin/metabolism Plasminogen Activators/metabolism
Chemicals
Fibrin Deoxyglucose Plasminogen Activators
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wolf B A
Goldberg A R
References (31)
31 references, click to expand
  1. Fibrinolytic activity in a human fibrosarcoma cell line and evidence for the induction of plasminogen activator secretion during tumor formation.
    Cell. 1975 Oct;6(2):245-52 PMID: 1182804
  2. Plasminogen: purification from human plasma by affinity chromatography.
    Science. 1970 Dec 4;170(3962):1095-6 PMID: 5475635
  3. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  4. Plasminogen-independent fibrinolysis by proteases produced by transformed chick embryo fibroblasts.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):1132-6 PMID: 165484
  5. Inhibition of protease activity in cultures of rous sarcoma virus-transformed cells: effect on the transformed phenotype.
    Cell. 1975 Jul;5(3):253-61 PMID: 167981
  6. Purification of DNA complementary to nucleotide sequences required for neoplastic transformation of fibroblasts by avian sarcoma viruses.
    J Mol Biol. 1976 Mar 5;101(3):349-65 PMID: 176368
  7. A difference in the architecture of the surface membrane of normal and virally transformed cells.
    Proc Natl Acad Sci U S A. 1969 Mar;62(3):994-1001 PMID: 4308100
  8. Rous sarcoma virus: a function required for the maintenance of the transformed state.
    Nature. 1970 Sep 5;227(5262):1021-3 PMID: 4317808
  9. Analysis of a functional change in membrane in the process of cell transformation by Rous sarcoma virus; alteration in the characteristics of sugar transport.
    Virology. 1970 Aug;41(4):647-52 PMID: 4319783
  10. Differences between the ribonucleic acids of transforming and nontransforming avian tumor viruses.
    Proc Natl Acad Sci U S A. 1970 Dec;67(4):1673-80 PMID: 4321342
  11. Conditional lethal mutants of avian sarcoma viruses. I. Physiology of ts 75 and ts 149.
    Virology. 1971 Feb;43(2):375-89 PMID: 4322624
  12. [Isolation and study of a conditional mutant of Rous virus with thermosensitive transforming capacity].
    C R Acad Sci Hebd Seances Acad Sci D. 1970 Dec 21;271(25):2430-3 PMID: 4324407
  13. Induction of mutations in an RNA tumour virus by an analogue of a DNA precursor.
    Nat New Biol. 1971 Nov 3;234(44):11-2 PMID: 4330167
  14. Temperature-dependent alterations in sugar transport in cells infected by a temperature-sensitive mutant of Rous sarcoma virus.
    Proc Natl Acad Sci U S A. 1971 Nov;68(11):2739-41 PMID: 4330938
  15. The effects of reciprocal changes in temperature on the transformed state of cells infected with a rous sarcoma virus mutant.
    Virology. 1971 Nov;46(2):470-9 PMID: 4331732
  16. Selective inhibition of growth of transformed cells by protease inhibitors.
    Proc Natl Acad Sci U S A. 1972 Dec;69(12):3825-7 PMID: 4345510
  17. An enzymatic function associated with transformation of fibroblasts by oncogenic viruses. II. Mammalian fibroblast cultures transformed by DNA and RNA tumor viruses.
    J Exp Med. 1973 Jan 1;137(1):112-26 PMID: 4347288
  18. An enzymatic function associated with transformation of fibroblasts by oncogenic viruses. I. Chick embryo fibroblast cultures transformed by avian RNA tumor viruses.
    J Exp Med. 1973 Jan 1;137(1):85-111 PMID: 4347290
  19. Alteration of cell-surface proteins by viral transformation and by proteolysis.
    Proc Natl Acad Sci U S A. 1973 Nov;70(11):3170-4 PMID: 4361679
  20. Non-selective inhibition of transformed cell growth by a protease inhibitor.
    Proc Natl Acad Sci U S A. 1974 May;71(5):1748-52 PMID: 4365571
  21. Fibrinolysis associated with oncogenic transformation. Partial purification and characterization of the cell factor, a plasminogen activator.
    J Biol Chem. 1974 Jul 10;249(13):4295-305 PMID: 4368727
  22. Plasminogen, the serum proenzyme activated by factors from cells transformed by oncogenic viruses.
    J Biol Chem. 1974 Jul 10;249(13):4306-11 PMID: 4369021
  23. Plasminogen activator production accompanies loss of anchorage regulation in transformation of primary rat embryo cells by simian virus 40.
    Proc Natl Acad Sci U S A. 1974 Dec;71(12):4792-6 PMID: 4373730
  24. Increased protease levels in transformed cells: a casein overlay assay for the detection of plasminogen activator production.
    Cell. 1974 Jun;2(2):95-102 PMID: 4376055
  25. Cellular tumorigenicity in nude mice: correlation with cell growth in semi-solid medium.
    Cell. 1974 Dec;3(4):355-9 PMID: 4442124
  26. Involvement of cell and serum proteases in exposing tumor-specific agglutinin sites.
    Ann N Y Acad Sci. 1974;234(0):348-54 PMID: 4528615
  27. Effects of protease inhibitors on growth of hamster tumor cells in culture.
    Cancer Res. 1972 Nov;32(11):2469-74 PMID: 4628323
  28. Tumor viruses: 1974.
    Cold Spring Harb Symp Quant Biol. 1975;39 Pt 2:1187-200 PMID: 50896
  29. Proteolytic enzymes initiating cell division and escape from contact inhibition of growth.
    Nature. 1970 Jul 11;227(5254):170-1 PMID: 5428405
  30. Release from density dependent growth inhibition by proteolytic enzymes.
    Nature. 1970 Aug 22;227(5260):843-5 PMID: 5464581
  31. The polymerization of proteins; the action of thrombin on fibrinogen.
    Arch Biochem Biophys. 1951 Jul;32(2):317-24 PMID: 14857750
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.73.10.3613
Published
1976-10-00
Pages
3613-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC431168
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.73.10.3613 PMC Full Text View journal details
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