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PMID: 18557819 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

SPOROCYTELESS modulates YUCCA expression to regulate the development of lateral organs in Arabidopsis.

The New phytologist ·Vol. 179 ·No. 3 ·2008-00-00 ·Pages 751-764

Li LC, Qin GJ, Tsuge T, Hou XH, Ding MY, Aoyama T, Oka A, Chen Z, Gu H, Zhao Y, Qu LJ

Abstract

* Auxin is essential for many aspects of plant growth and development, including the determination of lateral organ shapes. * Here, the characterization of a dominant Arabidopsis thaliana mutant spl-D (SPOROCYTELESS dominant), and the roles of SPL in auxin homeostasis and plant development, are reported. * The spl-D mutant displayed a severe up-curling leaf phenotype caused by increased expression of SPOROCYTELESS/NOZZLE (SPL/NZZ), a putative transcription factor gene that was previously linked to sporocyte formation. The spl-D plants also displayed pleiotropic developmental defects including fewer lateral roots, simpler venation patterns, and reduced shoot apical dominance. The leaf and floral phenotypes of spl-D and SPL over-expression lines were reminiscent of yucca (yuc) triple and quadruple mutants, suggesting that SPL may regulate auxin homeostasis. Consistent with this hypothesis, it was found that over-expression of SPL led to down-regulation of the auxin reporter DR5-GUS, and that many auxin-responsive genes were down-regulated in spl-D leaves. Interestingly, the expression of YUC2 and YUC6, two key genes in auxin biosynthesis, was significantly repressed in spl-D plants. * Taken together with the genetic and phenotypic analysis of spl-D/yuc6-D double mutant, these data suggest that SPL may regulate auxin homeostasis by repressing the transcription of YUC2 and YUC6 and participate in lateral organ morphogenesis.

MeSH Terms
Arabidopsis/anatomy & histology,genetics,growth & development Arabidopsis Proteins/biosynthesis,genetics,physiology Cloning, Molecular Gene Expression Regulation, Plant Homeostasis Indoleacetic Acids/metabolism Mutagenesis, Insertional Nuclear Proteins/genetics,physiology Oxygenases/biosynthesis,genetics Phenotype Plant Leaves/anatomy & histology,genetics,growth & development Plants, Genetically Modified/growth & development,metabolism Repressor Proteins/genetics,physiology
Chemicals
Arabidopsis Proteins Indoleacetic Acids Nuclear Proteins Repressor Proteins SPL protein, Arabidopsis Oxygenases YUC protein, Arabidopsis
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Li Lin-Chuan
National Laboratory for Protein Engineering and Plant Genetic Engineering, Peking-Yale Joint Research Center for Plant Molecular Genetics and AgroBiotechnology, College of Life Sciences, Peking University, Beijing 100871, China.
Qin Gen-Ji
National Laboratory for Protein Engineering and Plant Genetic Engineering, Peking-Yale Joint Research Center for Plant Molecular Genetics and AgroBiotechnology, College of Life Sciences, Peking University, Beijing 100871, China.
Tsuge Tomohiko
Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011, Japan.
Hou Xian-Hui
National Laboratory for Protein Engineering and Plant Genetic Engineering, Peking-Yale Joint Research Center for Plant Molecular Genetics and AgroBiotechnology, College of Life Sciences, Peking University, Beijing 100871, China.
Ding Mao-Yu
National Laboratory for Protein Engineering and Plant Genetic Engineering, Peking-Yale Joint Research Center for Plant Molecular Genetics and AgroBiotechnology, College of Life Sciences, Peking University, Beijing 100871, China.
Aoyama Takashi
Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011, Japan.
Oka Atsuhiro
Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011, Japan.
Chen Zhangliang
National Laboratory for Protein Engineering and Plant Genetic Engineering, Peking-Yale Joint Research Center for Plant Molecular Genetics and AgroBiotechnology, College of Life Sciences, Peking University, Beijing 100871, China.
Gu Hongya
National Laboratory for Protein Engineering and Plant Genetic Engineering, Peking-Yale Joint Research Center for Plant Molecular Genetics and AgroBiotechnology, College of Life Sciences, Peking University, Beijing 100871, China. | The National Plant Gene Research Center (Beijing), Beijing 100101, China.
Zhao Yunde
Section of Cell and Developmental Biology, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0116, USA.
Qu Li-Jia
National Laboratory for Protein Engineering and Plant Genetic Engineering, Peking-Yale Joint Research Center for Plant Molecular Genetics and AgroBiotechnology, College of Life Sciences, Peking University, Beijing 100871, China. | The National Plant Gene Research Center (Beijing), Beijing 100101, China.
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Article Info
Journal
The New phytologist
Abbr.
New Phytol
ISSN
1469-8137
DOI
10.1111/j.1469-8137.2008.02514.x
Published
2008-00-00
Epub
2008-00-28
Pages
751-764
Language
English
Region
England
NLM ID
9882884
Subset
IM
Grants
NIGMS NIH HHS · R01 GM068631 · United States
External Links
PubMed source DOI: 10.1111/j.1469-8137.2008.02514.x View journal details
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