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PMID: 185322 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Synthesis of Sendai virus polypeptides by a cell-free extract from wheat germ.

The Journal of general virology ·Vol. 33 ·No. 1 ·1976-10-00 ·Pages 117-23

Davies JW, Portner A, Kingsbury DW

Abstract

The '18S' RNA population from Sendai virus-infected cells efficiently directed the synthesis of three virus-specific polypeptides P, NP and M, in wheat germ cell-free extracts. In agreement with previous results obtained with a reticulocyte extract, there was little or no production of virus glycopolypeptides. Analyses of tryptic peptides revealed close correspondence between the primary structures of NP and M made in vitro and the authentic virus polypeptides.

MeSH Terms
Cell-Free System Glycopeptides/biosynthesis Leucine/metabolism Mosaic Viruses/metabolism Parainfluenza Virus 1, Human/metabolism Peptide Biosynthesis Plant Extracts Protein Biosynthesis RNA, Messenger/metabolism RNA, Viral/metabolism Triticum Viral Proteins/biosynthesis
Chemicals
Glycopeptides Plant Extracts RNA, Messenger RNA, Viral Viral Proteins Leucine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Davies J W
Portner A
Kingsbury D W
Article Info
Journal
The Journal of general virology
Abbr.
J Gen Virol
ISSN
0022-1317
Published
1976-10-00
Pages
117-23
Language
English
Region
England
NLM ID
0077340
Subset
IM
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