Structure of the human type I DNA topoisomerase gene.

The Journal of biological chemistry ·Vol. 266 ·No. 15 ·1991-05-25 ·Pages 9610-6

Kunze N, Yang GC, Dölberg M, Sundarp R, Knippers R, Richter A

Abstract

We describe the molecular organization of the human gene coding for type I DNA topoisomerase. The coding sequence is split into 21 exons distributed over at least 85 kilobase pairs (kb) of human genomic DNA. The sizes of the 20 introns vary widely between 0.2 and at least 30 kb and all contain the sequence elements known to be required for pre-mRNA splicing. Several of the intron sequences separate exons encoding parts of the enzyme that are highly conserved between human and yeast suggesting that at least some of the exons may code for individual, structurally, or functionally important domains of the enzyme. We also describe the promoter sequence of the human topoisomerase I gene and show that it is composed of distinct functional elements.

Related Genes

hTOP1

MeSH Terms

Amino Acid Sequence Base Sequence DNA/genetics DNA Topoisomerases, Type I/genetics Exons HeLa Cells Humans Introns Lymphocytes/metabolism Molecular Sequence Data Promoter Regions, Genetic Restriction Mapping Sequence Alignment

Chemicals

DNA DNA Topoisomerases, Type I

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Kunze N

Division of Biology, University of Konstanz, Germany.

Yang G C

Dölberg M

Sundarp R

Knippers R

Richter A

Article Info

Journal

The Journal of biological chemistry

Abbr.

J Biol Chem

ISSN

0021-9258

Published

1991-05-25

Pages

9610-6

Language

English

Region

United States

NLM ID

2985121R

Subset

Databases

GENBANK

M60688, M60689, M60690, M60691, M60692, M60693, M60694, M60695, M60696, M60697, M60698, M60699, M60700, M60701, M60702, M60703, M60704, M60705, M60706

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Structure of the human type I DNA topoisomerase gene.

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