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PMID: 1851530 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Ligand autoradiographic receptor screening. II. Expression of receptor cDNA in transfected COS cells grown on polyester disks and its recovery.

Brain research. Molecular brain research ·Vol. 9 ·No. 3 ·1991-02-00 ·Pages 271-6

Schaeffer JC, Lin CL, Kitayama S, Uhl GR

Abstract

A receptor autoradiographic method that can detect expressed receptor cDNA in 0.002% of plasmid clones and can allow cDNA recoveries and enrichments up to 300-fold in one cycle of screening is described.

MeSH Terms

Animals Autoradiography Cell Line, Transformed DNA/biosynthesis,isolation & purification Ligands Polyesters Receptors, Cell Surface/genetics Reproducibility of Results Transfection/genetics

Chemicals

Ligands Polyesters Receptors, Cell Surface DNA

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Schaeffer J C

Laboratory of Molecular Neurobiology, ARC/NIDA, Baltimore, MD 21224.

Lin C L

Kitayama S

Uhl G R

Article Info

Journal

Brain research. Molecular brain research

Abbr.

Brain Res Mol Brain Res

ISSN

0169-328X

DOI

10.1016/0169-328x(91)90012-m

Published

1991-02-00

Pages

271-6

Language

English

Region

Netherlands

NLM ID

8908640

Subset

External Links

PubMed source DOI: 10.1016/0169-328x(91)90012-m

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Ligand autoradiographic receptor screening. II. Expression of receptor cDNA in transfected COS cells grown on polyester disks and its recovery.

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