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PMID: 1850969 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular characterization of the class II multiresistance transposable element Tn1403 from Pseudomonas aeruginosa.

Antimicrobial agents and chemotherapy ·Vol. 35 ·No. 2 ·1991-02-00 ·Pages 313-21

Vézina G, Levesque RC

Abstract

Transposon Tn1403 is a 19.9-kb multiresistance class II transposable element originally found on the RPL11 plasmid from a clinical isolate of Pseudomonas aeruginosa. It encodes resistance to ampicillin (PSE-1 beta-lactamase), streptomycin and spectinomycin (aadA and aphC), and chloramphenicol (cat). It has structural homology with the tnpM and tnpI sequences of Tn21 and inverted repeats and res and tnpR sequences of Tn501, but it has no structural homology nor functional complementation with the resolvase gene of Tn21 or Tn3. Sequence analysis revealed long inverted repeats at each extremity of Tn1403 containing 38-bp inverted repeats that were 97.4% similar to those of Tn1721 and 5-bp direct repeats. Transposition assays showed a low frequency of transposition (3.5 x 10(-6)) compared with that of Tn3 (3.3 x 10(-3)) and no resolution of cointegrates.

MeSH Terms
Base Sequence Conjugation, Genetic DNA Transposable Elements DNA, Bacterial/chemistry,immunology Drug Resistance, Microbial/genetics Electrophoresis, Polyacrylamide Gel Molecular Sequence Data Nucleic Acid Hybridization Oligonucleotides/chemical synthesis Plasmids Pseudomonas aeruginosa/genetics Transposon Resolvases
Chemicals
DNA Transposable Elements DNA, Bacterial Oligonucleotides Tn21 resolvase Transposon Resolvases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Vézina G
Département de Microbiologie, Faculté de Médecine, Université Laval, Québec, Canada.
Levesque R C
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.35.2.313
Published
1991-02-00
Pages
313-21
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC244998
Subset
IM
Databases
GENBANK
M59034, M59035, M59036
External Links
PubMed source DOI: 10.1128/AAC.35.2.313 PMC Full Text View journal details
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