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PMID: 18491976 Published · ppublish English Journal Article Research Support, N.I.H., Intramural Research Support, Non-U.S. Gov't

Development of real-time in vivo imaging of device-related Staphylococcus epidermidis infection in mice and influence of animal immune status on susceptibility to infection.

The Journal of infectious diseases ·Vol. 198 ·No. 2 ·2008-07-15 ·Pages 258-61

Vuong C, Kocianova S, Yu J, Kadurugamuwa JL, Otto M

Abstract

Staphylococcus epidermidis is the leading cause of hospital-acquired device-related infections, but there is a lack of suitable methods to investigate the pathogenesis of S. epidermidis infection. We created a bioluminescent strain of S. epidermidis and developed a subcutaneous catheter-related murine infection model for real-time monitoring of biofilm-associated infection. Additionally, we compared severely immunocompromised and immunocompetent mice, demonstrating the substantial effect of animal immune status on susceptibility to experimentally induced S. epidermidis disease. This study presents a novel approach for investigating the in vivo details of the pathogenesis of S. epidermidis infection.

MeSH Terms
Animals Biofilms Colony-Forming Units Assay Disease Susceptibility/immunology Female Luminescence Mice Mice, SCID Staphylococcal Infections/diagnosis,immunology Staphylococcus epidermidis T-Lymphocytes/immunology T-Lymphocytes, Cytotoxic/immunology
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Vuong Cuong
Department of Anesthesiology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA. cuong.vuong@umass.med.edu
Kocianova Stanislava
Yu Jun
Kadurugamuwa Jagath L
Otto Michael
References (31)
31 references, click to expand
  1. In vivo bioluminescence imaging.
    Comp Med. 2004 Dec;54(6):631-4 PMID: 15679260
  2. Characterization of the importance of polysaccharide intercellular adhesin/hemagglutinin of Staphylococcus epidermidis in the pathogenesis of biomaterial-based infection in a mouse foreign body infection model.
    Infect Immun. 1999 May;67(5):2627-32 PMID: 10225932
  3. Staphylococcus epidermidis: an increasing cause of infection in patients with granulocytopenia.
    Ann Intern Med. 1982 Oct;97(4):503-8 PMID: 7125409
  4. New aspects of staphylococcal infections: emergence of coagulase-negative staphylococci as pathogens.
    Antonie Van Leeuwenhoek. 1984;50(5-6):729-44 PMID: 6397138
  5. In vivo bioluminescence imaging of the murine pathogen Citrobacter rodentium.
    Infect Immun. 2006 Sep;74(9):5391-6 PMID: 16926434
  6. Biofilm formation in medical device-related infection.
    Int J Artif Organs. 2006 Apr;29(4):343-59 PMID: 16705603
  7. In vivo models to evaluate adhesion and biofilm formation by Staphylococcus epidermidis.
    Methods Enzymol. 2001;336:206-15 PMID: 11398400
  8. Rapid direct method for monitoring antibiotics in a mouse model of bacterial biofilm infection.
    Antimicrob Agents Chemother. 2003 Oct;47(10):3130-7 PMID: 14506020
  9. Characterization of Staphylococcus epidermidis polysaccharide intercellular adhesin/hemagglutinin in the pathogenesis of intravascular catheter-associated infection in a rat model.
    Infect Immun. 1999 May;67(5):2656-9 PMID: 10225938
  10. Direct continuous method for monitoring biofilm infection in a mouse model.
    Infect Immun. 2003 Feb;71(2):882-90 PMID: 12540570
  11. Bacterial colonization and the expression of inducible nitric oxide synthase in murine wounds.
    Am J Pathol. 2002 Dec;161(6):2143-52 PMID: 12466130
  12. Key role of poly-gamma-DL-glutamic acid in immune evasion and virulence of Staphylococcus epidermidis.
    J Clin Invest. 2005 Mar;115(3):688-94 PMID: 15696197
  13. Increased colonization of indwelling medical devices by quorum-sensing mutants of Staphylococcus epidermidis in vivo.
    J Infect Dis. 2004 Oct 15;190(8):1498-505 PMID: 15378444
  14. Molecular imaging using visible light to reveal biological changes in the brain.
    Neuroimaging Clin N Am. 2006 Nov;16(4):633-54, ix PMID: 17148024
  15. Pathogenesis of infections due to coagulase-negative staphylococci.
    Lancet Infect Dis. 2002 Nov;2(11):677-85 PMID: 12409048
  16. Extracellular replication of Listeria monocytogenes in the murine gall bladder.
    Science. 2004 Feb 6;303(5659):851-3 PMID: 14764883
  17. Laboratory, clinical, and epidemiological aspects of coagulase-negative staphylococci.
    Clin Microbiol Rev. 1988 Jul;1(3):281-99 PMID: 3058297
  18. Staphylococcus epidermidis infections.
    Microbes Infect. 2002 Apr;4(4):481-9 PMID: 11932199
  19. Experimental neosporosis in bulls: parasite detection in semen and blood and specific antibody and interferon-gamma responses.
    Theriogenology. 2007 Apr 1;67(6):1175-84 PMID: 17316779
  20. Applications of bioluminescence imaging to the study of infectious diseases.
    Cell Microbiol. 2007 Oct;9(10):2315-22 PMID: 17587328
  21. A crucial role for exopolysaccharide modification in bacterial biofilm formation, immune evasion, and virulence.
    J Biol Chem. 2004 Dec 24;279(52):54881-6 PMID: 15501828
  22. Advances in in vivo bioluminescence imaging of gene expression.
    Annu Rev Biomed Eng. 2002;4:235-60 PMID: 12117758
  23. T-dependent production and activation of mononuclear phagocytes during murine BCG infection.
    Immunol Lett. 1985;11(3-4):189-94 PMID: 3910568
  24. Response of congenitally athymic (nude) mice to infection with Mycobacterium bovis (strain BCG).
    J Natl Cancer Inst. 1975 Jun;54(6):1419-26 PMID: 1094126
  25. T cells are essential for bacterial clearance, and gamma interferon, tumor necrosis factor alpha, and B cells are crucial for disease development in Coxiella burnetii infection in mice.
    Infect Immun. 2007 Jul;75(7):3245-55 PMID: 17438029
  26. Opsonic defense to Staphylococcus epidermidis in the premature neonate.
    J Infect Dis. 1985 Nov;152(5):930-7 PMID: 4045255
  27. Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228).
    Mol Microbiol. 2003 Sep;49(6):1577-93 PMID: 12950922
  28. Experimental infection of severe combined immunodeficient beige mice with Mycobacterium paratuberculosis of bovine origin.
    Infect Immun. 1992 Oct;60(10):4074-9 PMID: 1398920
  29. Parallel induction by glucose of adherence and a polysaccharide antigen specific for plastic-adherent Staphylococcus epidermidis: evidence for functional relation to intercellular adhesion.
    Infect Immun. 1992 May;60(5):2048-57 PMID: 1314224
  30. Antibacterial effect of human V gamma 2V delta 2 T cells in vivo.
    J Clin Invest. 2001 Nov;108(9):1349-57 PMID: 11696580
  31. Cryptococcus neoformans var. grubii isolates recovered from persons with AIDS demonstrate a wide range of virulence during murine meningoencephalitis that correlates with the expression of certain virulence factors.
    Microbiology (Reading). 2006 Aug;152(Pt 8):2247-2255 PMID: 16849791
Article Info
Journal
The Journal of infectious diseases
Abbr.
J Infect Dis
ISSN
0022-1899
DOI
10.1086/589307
Email
cuong.vuong@umass.med.edu
Published
2008-07-15
Pages
258-61
Language
English
Region
United States
NLM ID
0413675
PMCID
PMC2763270
Subset
IM
Grants
Intramural NIH HHS · Z01 AI000904-06 · United States
Corrections
ErratumIn
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External Links
PubMed source DOI: 10.1086/589307 PMC Full Text View journal details
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