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PMID: 1849141 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

92-kD type IV collagenase mediates invasion of human cytotrophoblasts.

The Journal of cell biology ·Vol. 113 ·No. 2 ·1991-04-00 ·Pages 437-49

Librach CL, Werb Z, Fitzgerald ML, Chiu K, Corwin NM, Esteves RA, Grobelny D, Galardy R, Damsky CH, Fisher SJ

Abstract

The specialized interaction between embryonic and maternal tissues is unique to mammalian development. This interaction begins with invasion of the uterus by the first differentiated embryonic cells, the trophoblasts, and culminates in formation of the placenta. The transient tumor-like behavior of cytotrophoblasts, which peaks early in pregnancy, is developmentally regulated. Likewise, in culture only early-gestation human cytotrophoblasts invade a basement membrane-like substrate. These invasive cells synthesize both metalloproteinases and urokinase-type plasminogen activator. Metalloproteinase inhibitors and a function-perturbing antibody specific for the 92-kD type IV collagen-degrading metalloproteinase completely inhibited cytotrophoblast invasion, whereas inhibitors of the plasminogen activator system had only a partial (20-40%) inhibitory effect. We conclude that the 92-kD type IV collagenase is critical for cytotrophoblast invasion.

MeSH Terms
Antibody Specificity Basement Membrane/metabolism Cell Aggregation Cells, Cultured Embryonic and Fetal Development Female Humans Matrix Metalloproteinase 9 Microbial Collagenase/antagonists & inhibitors,immunology,metabolism Microscopy, Electron, Scanning Photomicrography Pregnancy Trophoblasts/metabolism,ultrastructure
Chemicals
Microbial Collagenase Matrix Metalloproteinase 9
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Librach C L
Department of Stomatology, University of California, San Francisco 94143.
Werb Z
Fitzgerald M L
Chiu K
Corwin N M
Esteves R A
Grobelny D
Galardy R
Damsky C H
Fisher S J
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.113.2.437
Published
1991-04-00
Pages
437-49
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2288933
Subset
IM
Grants
NICHD NIH HHS · HD 22518 · United States
NICHD NIH HHS · HD 23539 · United States
NICHD NIH HHS · P01 HD 2418 · United States
External Links
PubMed source DOI: 10.1083/jcb.113.2.437 PMC Full Text View journal details
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