Home LiteratureArticle Details

PMID: 1846721 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Demonstration of the genuine iso-12p character of the standard marker chromosome of testicular germ cell tumors and identification of further chromosome 12 aberrations by competitive in situ hybridization.

American journal of human genetics ·Vol. 48 ·No. 2 ·1991-02-00 ·Pages 269-73

Suijkerbuijk RF, van de Veen AY, van Echten J, Buys CH, de Jong B, Oosterhuis JW, Warburton DA, Cassiman JJ, Schonk D, Geurts van Kessel A

Abstract

The recently developed competitive in situ hybridization (CISH) strategy was applied to the analysis of chromosome 12 aberrations in testicular germ cell tumors (TGCTs). DNAs from two rodent-human somatic cell hybrids, containing either a normal chromosome 12 or the p arm of chromosome 12 as their unique human material, were used as probes. Our results demonstrate a genuine iso-12p character of the standard marker chromosome in TGCTs. Moreover, variant markers were identified representing translocation products that also involve chromosome 12.

MeSH Terms

Chromosome Aberrations Chromosomes, Human, Pair 12 DNA Probes DNA, Neoplasm/genetics Genetic Markers Humans Immunohistochemistry Karyotyping Male Neoplasms, Germ Cell and Embryonal/genetics Nucleic Acid Hybridization Testicular Neoplasms/genetics Translocation, Genetic Tumor Cells, Cultured

Chemicals

DNA Probes DNA, Neoplasm Genetic Markers

Authors & Affiliations

10 authors, click to expand affiliations / ORCID

Suijkerbuijk R F

Department of Human Genetics, University Hospital Nijmegen, The Netherlands.

van de Veen A Y

van Echten J

Buys C H

de Jong B

Oosterhuis J W

Warburton D A

Cassiman J J

Schonk D

Geurts van Kessel A

References (14)

14 references, click to expand

Leukemic differentiation of a mediastinal germ cell tumor.
Genes Chromosomes Cancer. 1989 Sep;1(1):83-7 PMID: 2562115
Localization and polymorphism of a chromosome 12-specific alpha satellite DNA sequence.
Cytogenet Cell Genet. 1990;53(4):216-8 PMID: 1976486
i(12p)-negative testicular germ cell tumors. A different group?
Cancer Genet Cytogenet. 1988 Oct 15;35(2):171-8 PMID: 2846146
Chromosome changes in germ cell tumors of the testis.
Cancer Genet Cytogenet. 1986 Jan 15;19(3-4):245-52 PMID: 3002602
Chromosome 12q heterozygosity is retained in i(12p)-positive testicular germ cell tumor cells.
Cancer Genet Cytogenet. 1989 Jul 1;40(1):129-34 PMID: 2569358
Molecular analysis of the isochromosome 12P in the Pallister-Killian syndrome. Construction of a mouse-human hybrid cell line containing an i(12p) as the sole human chromosome.
Hum Genet. 1989 Nov;83(4):359-63 PMID: 2807276
i(12p): specific chromosomal marker in seminoma and malignant teratoma of the testis?
Cancer Genet Cytogenet. 1983 Oct;10(2):199-204 PMID: 6616439
Cloned human teratoma cells differentiate into neuron-like cells and other cell types in retinoic acid.
J Cell Sci. 1984 Dec;72:37-64 PMID: 6085330
A chromosome study of three ovarian tumors.
Cancer Genet Cytogenet. 1987 Jun;26(2):327-37 PMID: 3471312
Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization.
Proc Natl Acad Sci U S A. 1986 May;83(9):2934-8 PMID: 3458254
Cytogenetics of carcinoma in situ of the testis.
Cancer Genet Cytogenet. 1990 May;46(1):75-81 PMID: 2331686
Monochromosomal rodent-human hybrids from microcell fusion of human lymphoblastoid cells containing an inserted dominant selectable marker.
Genomics. 1990 Feb;6(2):358-66 PMID: 2307476
Direct nonradioactive in situ hybridization of somatic cell hybrid DNA to human lymphocyte chromosomes.
Cytometry. 1990;11(1):105-9 PMID: 2307050
Isochromosome 12p in mediastinal germ cell tumor.
Cancer Genet Cytogenet. 1989 Oct 15;42(2):243-51 PMID: 2790759

Article Info

Journal

American journal of human genetics

Abbr.

Am J Hum Genet

ISSN

0002-9297

Published

1991-02-00

Pages

269-73

Language

English

Region

United States

NLM ID

0370475

PMCID

PMC1683009

Subset

External Links

PubMed source PMC Full Text View journal details

Analysis Services

Demonstration of the genuine iso-12p character of the standard marker chromosome of testicular germ cell tumors and identification of further chromosome 12 aberrations by competitive in situ hybridization.

Contact

WeChat Official Account