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PMID: 1846665 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Transformation-defective v-ski induces MyoD and myogenin expression but not myotube formation.

Molecular and cellular biology ·Vol. 11 ·No. 2 ·1991-02-00 ·Pages 1167-70

Colmenares C, Teumer JK, Stavnezer E

Abstract

The ski oncogene induces muscle differentiation in otherwise nonmyogenic quail embryo cells (C. Colmenares and E. Stavnezer, Cell 59:293-303, 1989). Here we report that v-ski induces both MyoD and myogenin expression, suggesting that activation of these muscle regulatory genes may be a critical step in ski-induced myogenesis. We also describe a transformation-defective mutant of v-ski (tdM5i) that fails to induce myotube formation, although it induces the expression of many muscle-specific genes, including the MyoD and myogenin genes. Therefore, if activation of MyoD and myogenin expression is a necessary component of the myogenic program triggered by ski, it is clearly insufficient to account for complete muscle differentiation.

Related Genes
v-ski
MeSH Terms
Amino Acid Sequence Animals Cell Differentiation Cell Division Cells, Cultured DNA-Binding Proteins/genetics Embryo, Nonmammalian Molecular Sequence Data Muscle Proteins/genetics Muscles/cytology,metabolism Mutagenesis, Site-Directed MyoD Protein Myogenin Oncogenes Quail Trans-Activators/genetics
Chemicals
DNA-Binding Proteins Muscle Proteins MyoD Protein Myogenin Trans-Activators
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Colmenares C
Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati Medical Center, Ohio 45267-0524.
Teumer J K
Stavnezer E
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28 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.11.2.1167-1170.1991
Published
1991-02-00
Pages
1167-70
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC359802
Subset
IM
Grants
NCI NIH HHS · CA43600 · United States
External Links
PubMed source DOI: 10.1128/mcb.11.2.1167-1170.1991 PMC Full Text View journal details
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