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PMID: 18465222 Published · ppublish English Comparative Study Journal Article

Glycan structures of ocular surface mucins in man, rabbit and dog display species differences.

Glycoconjugate journal ·Vol. 25 ·No. 8 ·2008-11-00 ·Pages 763-73

Royle L, Matthews E, Corfield A, Berry M, Rudd PM, Dwek RA, Carrington SD

Abstract

The composition of the mucus gel of the tear film reflects the competing needs for transparency, stability, hydration, and protection of the ocular surface. Mucins form the macromolecular scaffolding of this hydrated gel, and glycans decorating these glycoproteins represent a rich source of binding ligands that may both modulate microbial binding and regulate the physicochemical characteristics of the gel. This study compares the structure of O-linked glycans derived from the ocular mucins of three species, to determine whether the ocular surface microenvironment dictates the need for a common pattern of O-linked carbohydrate structures. Ocular mucus aspirates were collected from healthy humans, rabbits and dogs. Mucins were purified using standard protocols. O-glycans were released by hydrazinoloysis and subsequently analysed by a combination of HPLC, exoglycosidase digestions and LC-MS/MS. A total of 12 different O-glycans were identified. In human ocular mucin, the majority were negatively charged and terminated in sialic acid, whilst those from rabbit or dog were mainly neutral and terminated in alpha 1-2 fucose and/or alpha 1-3 N-acetylgalactosamine. The glycans were short: the most common structures being tetra-, tri- or disaccharides. Less elaborate glycan structures are encountered at the ocular surface than at many other mucosal surfaces. Species-specific glycan expression is a feature of ocular surface mucins, and has implications for their defensive properties where different microbial and environmental challenges are encountered.

MeSH Terms
Adolescent Adult Aged Animals Carbohydrate Sequence Chromatography, High Pressure Liquid Conjunctiva/chemistry Dogs Female Glycosylation Humans Male Middle Aged Molecular Sequence Data Molecular Structure Mucins/chemistry Polysaccharides/chemistry Rabbits Species Specificity Tears/chemistry Young Adult
Chemicals
Mucins Polysaccharides
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Royle Louise
Department of Biochemistry, Glycobiology Institute, University of Oxford, South Parks Road, Oxford, OX1 3QU, UK.
Matthews Elizabeth
Corfield Anthony
Berry Monica
Rudd Pauline M
Dwek Raymond A
Carrington Stephen D
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Article Info
Journal
Glycoconjugate journal
Abbr.
Glycoconj J
ISSN
0282-0080
DOI
10.1007/s10719-008-9136-6
Published
2008-11-00
Epub
2008-00-09
Pages
763-73
Language
English
Region
United States
NLM ID
8603310
Subset
IM
External Links
PubMed source DOI: 10.1007/s10719-008-9136-6 View journal details
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