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题目:: T cell enrichment by nonadherence to nylon.
作者:: Hathcock(K S)
状态:: 发布时间2008-04-24 , 更新时间 2016-10-21
期刊:: Curr Protoc Immunol
摘要:: Differential adherence properties of T cells, B cells, and accessory cells such as macrophages or other APC (antigen-presenting cells) can be employed to enrich for T cells. While the basis for this adherence is not understood, either nylon wool or Sephadex G-10 can be used as column matrices to which B cells and accessory cells will more readily adhere than T cells. Such procedures offer a convenient, although imprecise, means of enriching T cells through removal of accessory and B cells; use of nylon wool is preferred if both of the latter subsets are to be removed, while Sephadex is used when the goal is primarily to remove accessory cells.
语言:: eng
DOI:: 10.1002/0471142735.im0302s30

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