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PMID: 18417612 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The Rho GDI Rdi1 regulates Rho GTPases by distinct mechanisms.

Molecular biology of the cell ·Vol. 19 ·No. 7 ·2008-07-00 ·Pages 2885-96

Tiedje C, Sakwa I, Just U, Höfken T

Abstract

The small guanosine triphosphate (GTP)-binding proteins of the Rho family are implicated in various cell functions, including establishment and maintenance of cell polarity. Activity of Rho guanosine triphosphatases (GTPases) is not only regulated by guanine nucleotide exchange factors and GTPase-activating proteins but also by guanine nucleotide dissociation inhibitors (GDIs). These proteins have the ability to extract Rho proteins from membranes and keep them in an inactive cytosolic complex. Here, we show that Rdi1, the sole Rho GDI of the yeast Saccharomyces cerevisiae, contributes to pseudohyphal growth and mitotic exit. Rdi1 interacts only with Cdc42, Rho1, and Rho4, and it regulates these Rho GTPases by distinct mechanisms. Binding between Rdi1 and Cdc42 as well as Rho1 is modulated by the Cdc42 effector and p21-activated kinase Cla4. After membrane extraction mediated by Rdi1, Rho4 is degraded by a novel mechanism, which includes the glycogen synthase kinase 3beta homologue Ygk3, vacuolar proteases, and the proteasome. Together, these results indicate that Rdi1 uses distinct modes of regulation for different Rho GTPases.

MeSH Terms
Actins/metabolism Cell Cycle Cell Membrane/metabolism Cell Polarity GTP-Binding Proteins/metabolism Gene Deletion Gene Expression Regulation, Enzymologic Gene Expression Regulation, Fungal Glycogen Synthase Kinase 3/metabolism Glycogen Synthase Kinase 3 beta Guanine Nucleotide Dissociation Inhibitors/metabolism,physiology Proteasome Endopeptidase Complex/metabolism Saccharomyces cerevisiae/enzymology,genetics Saccharomyces cerevisiae Proteins/metabolism,physiology cdc42 GTP-Binding Protein/metabolism rho GTP-Binding Proteins/chemistry,metabolism
Chemicals
Actins Guanine Nucleotide Dissociation Inhibitors Rdi1 protein, S cerevisiae Saccharomyces cerevisiae Proteins Glycogen Synthase Kinase 3 beta Glycogen Synthase Kinase 3 Proteasome Endopeptidase Complex GTP-Binding Proteins RHO1 protein, S cerevisiae RHO4 protein, S cerevisiae cdc42 GTP-Binding Protein rho GTP-Binding Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Tiedje Christopher
Institute of Biochemistry, Christian Albrecht University, 24098 Kiel, Germany.
Sakwa Imme
Just Ursula
Höfken Thomas
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1939-4586
DOI
10.1091/mbc.e07-11-1152
Published
2008-07-00
Epub
2008-00-16
Pages
2885-96
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC2441673
Subset
IM
External Links
PubMed source DOI: 10.1091/mbc.e07-11-1152 PMC Full Text View journal details
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