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PMID: 1840921 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

A parsley 4CL-1 promoter fragment specifies complex expression patterns in transgenic tobacco.

The Plant cell ·Vol. 3 ·No. 5 ·1991-05-00 ·Pages 435-43

Hauffe KD, Paszkowski U, Schulze-Lefert P, Hahlbrock K, Dangl JL, Douglas CJ

Abstract

The 4CL-1 gene is one of two highly homologous parsley genes encoding 4-coumarate:coenzyme A ligase, a key enzyme of general phenylpropanoid metabolism. Expression of these genes is essential for the biosynthesis of both defense-related and developmentally required phenylpropanoid derivatives. We examined the developmental regulation of the 4CL-1 promoter by analyzing the expression of 4CL-1-beta-glucuronidase fusions in transgenic tobacco plants. A 597-base pair 4CL-1 promoter fragment specified histochemically detectable expression in a complex array of vegetative and floral tissues and cell types. The activity of a series of 5' deleted promoter fragments was analyzed in parsley protoplasts and transgenic tobacco plants. Deletions past -210 base pairs led to a drastic decline in beta-glucuronidase activity in protoplasts and loss of tissue-specific expression in transgenic tobacco. These results were put into the context of potential protein-DNA interactions by in vivo footprint analysis of the 4CL-1 promoter in parsley cells. Loss of promoter activity in parsley protoplasts and transgenic tobacco was correlated with the deletion or disruption of the distal portion of a large (100-base pair) footprinted region within the first 200 base pairs of the 4CL-1 promoter.

MeSH Terms
Base Composition Base Sequence Coenzyme A Ligases/genetics,metabolism DNA Gene Expression Regulation Genes, Plant Molecular Sequence Data Organ Specificity/genetics Plants/enzymology,genetics Plants, Genetically Modified Plants, Toxic Promoter Regions, Genetic Protoplasts Restriction Mapping Tobacco
Chemicals
DNA Coenzyme A Ligases 4-coumarate-CoA ligase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Hauffe K D
Department of Botany, University of British Columbia, Vancouver, Canada.
Paszkowski U
Schulze-Lefert P
Hahlbrock K
Dangl J L
Douglas C J
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.3.5.435
Published
1991-05-00
Pages
435-43
Language
English
Region
England
NLM ID
9208688
PMCID
PMC160012
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.3.5.435 PMC Full Text View journal details
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