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PMID: 18370145 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

ENU mutagenesis in mice.

Methods in molecular biology (Clifton, N.J.) ·Vol. 415 ·2008-00-00 ·Pages 1-16

Georgel P, Du X, Hoebe K, Beutler B

Abstract

Forward genetics has led to many "breakthrough" discoveries, and with the mouse genome almost fully sequenced, the creation of phenotypes through random germline mutagenesis has become an efficient means by which to find the function of yet undescribed genes. In this chapter, we will provide a practical guideline for performing germline mutagenesis in mice. In particular, we will focus on the application of this technology to identify genes that are essential to innate immune defense.

MeSH Terms

Animals Chromosome Mapping Disease Susceptibility Ethylnitrosourea/toxicity Female Genome Herpesviridae Infections/virology Macrophages, Peritoneal/drug effects,metabolism Male Mice Mice, Inbred C57BL Molecular Biology/methods Muromegalovirus/drug effects Mutagenesis/drug effects Pedigree Phenotype Signal Transduction/drug effects Toll-Like Receptors/metabolism

Chemicals

Toll-Like Receptors Ethylnitrosourea

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Georgel Philippe

Laboratoire d'Immunogénétique Moléculaire Humaine, Centre de Recherche en Immunologie et Hématologie, Faculté de Médecine, Strasbourg Cedex, France.

Du Xin

Hoebe Kasper

Beutler Bruce

Article Info

Journal

Methods in molecular biology (Clifton, N.J.)

Abbr.

Methods Mol Biol

ISSN

1064-3745

DOI

10.1007/978-1-59745-570-1_1

Published

2008-00-00

Pages

1-16

Language

English

Region

United States

NLM ID

9214969

Subset

External Links

PubMed source DOI: 10.1007/978-1-59745-570-1_1

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ENU mutagenesis in mice.

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