Home LiteratureArticle Details
PMID: 18369947 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Exocytosis of endothelial cells is regulated by N-ethylmaleimide-sensitive factor.

Methods in molecular biology (Clifton, N.J.) ·Vol. 440 ·2008-00-00 ·Pages 203-15

Yamakuchi M, Ferlito M, Morrell CN, Matsushita K, Fletcher CA, Cao W, Lowenstein CJ

Abstract

Endothelial exocytosis of granules is a rapid response to vascular injury. However, the molecular machinery that regulates exocytosis in endothelial cells is not well understood. Recently developed techniques have defined the endothelial proteins that control vesicle and granule trafficking in endothelial cells. These techniques have revealed that syntaxin 4, synaptobrevin 3, and N-ethylmaleimide-sensitive factor (NSF) play a critical role in endothelial granule exocytosis. Additional studies have shown that nitric oxide regulates exocytosis by chemically modifying NSF. Further characterization of the factors that regulate exocytosis will lead to novel treatments for vascular diseases such as myocardial infarction and stroke.

MeSH Terms
Animals Biological Assay/methods Cell Adhesion Cell Separation Cells, Cultured Endothelial Cells/chemistry,metabolism Enzyme-Linked Immunosorbent Assay Exocytosis Flow Cytometry HL-60 Cells Humans Immunoprecipitation Leukocyte Rolling Leukocytes/metabolism Mice Microscopy, Video N-Ethylmaleimide-Sensitive Proteins/genetics,isolation & purification,metabolism Nitric Oxide/metabolism Peptide Fragments/metabolism Qa-SNARE Proteins/metabolism Recombinant Fusion Proteins/metabolism Secretory Vesicles/metabolism Time Factors Vesicle-Associated Membrane Protein 3/metabolism tat Gene Products, Human Immunodeficiency Virus/metabolism
Chemicals
Peptide Fragments Qa-SNARE Proteins Recombinant Fusion Proteins Vesicle-Associated Membrane Protein 3 tat Gene Products, Human Immunodeficiency Virus Nitric Oxide N-Ethylmaleimide-Sensitive Proteins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Yamakuchi Munekazu
Department of Medicine, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Ferlito Marcella
Morrell Craig N
Matsushita Kenji
Fletcher Craig A
Cao Wangsen
Lowenstein Charles J
References (12)
12 references, click to expand
  1. A novel inhibitor of N-ethylmaleimide-sensitive factor decreases leukocyte trafficking and peritonitis.
    J Pharmacol Exp Ther. 2005 Jul;314(1):155-61 PMID: 15778265
  2. Regulation of Weibel-Palade body exocytosis.
    Trends Cardiovasc Med. 2005 Nov;15(8):302-8 PMID: 16297768
  3. The Weibel-Palade body: the storage granule for von Willebrand factor and P-selectin.
    Thromb Haemost. 1993 Jul 1;70(1):105-10 PMID: 7694385
  4. Sphingosine 1-phosphate activates Weibel-Palade body exocytosis.
    Proc Natl Acad Sci U S A. 2004 Aug 3;101(31):11483-7 PMID: 15273282
  5. Nitric oxide regulates exocytosis by S-nitrosylation of N-ethylmaleimide-sensitive factor.
    Cell. 2003 Oct 17;115(2):139-50 PMID: 14567912
  6. NEW CYTOPLASMIC COMPONENTS IN ARTERIAL ENDOTHELIA.
    J Cell Biol. 1964 Oct;23:101-12 PMID: 14228505
  7. A novel class of fusion polypeptides inhibits exocytosis.
    Mol Pharmacol. 2005 Apr;67(4):1137-44 PMID: 15608145
  8. Antibody to human leukocyte antigen triggers endothelial exocytosis.
    Proc Natl Acad Sci U S A. 2007 Jan 23;104(4):1301-6 PMID: 17229850
  9. N-ethylmaleimide-sensitive factor: a redox sensor in exocytosis.
    Biol Chem. 2006 Oct-Nov;387(10-11):1377-83 PMID: 17081110
  10. The biotin switch method for the detection of S-nitrosylated proteins.
    Sci STKE. 2001 Jun 12;2001(86):pl1 PMID: 11752655
  11. TAT-mediated protein transduction into mammalian cells.
    Methods. 2001 Jul;24(3):247-56 PMID: 11403574
  12. Membrane fusion and exocytosis.
    Annu Rev Biochem. 1999;68:863-911 PMID: 10872468
Article Info
Journal
Methods in molecular biology (Clifton, N.J.)
Abbr.
Methods Mol Biol
ISSN
1064-3745
DOI
10.1007/978-1-59745-178-9_15
Published
2008-00-00
Pages
203-15
Language
English
Region
United States
NLM ID
9214969
PMCID
PMC2846407
Subset
IM
Grants
NHLBI NIH HHS · P01 HL065608 · United States
NHLBI NIH HHS · R01 HL078635-04 · United States
NHLBI NIH HHS · P01 HL065608-090007 · United States
NHLBI NIH HHS · R01 HL078635 · United States
NHLBI NIH HHS · P01 HL056091 · United States
NHLBI NIH HHS · P01 HL056091-050003 · United States
NCRR NIH HHS · T32 RR007002 · United States
NHLBI NIH HHS · P01 HL056091-090003 · United States
NHLBI NIH HHS · R01 HL074061 · United States
NHLBI NIH HHS · K08 HL074945 · United States
External Links
PubMed source DOI: 10.1007/978-1-59745-178-9_15 PMC Full Text
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2