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PMID: 18369186 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Review

Splicing regulation: from a parts list of regulatory elements to an integrated splicing code.

RNA (New York, N.Y.) ·Vol. 14 ·No. 5 ·2008-05-00 ·Pages 802-13

Wang Z, Burge CB

Abstract

Alternative splicing of pre-mRNAs is a major contributor to both proteomic diversity and control of gene expression levels. Splicing is tightly regulated in different tissues and developmental stages, and its disruption can lead to a wide range of human diseases. An important long-term goal in the splicing field is to determine a set of rules or "code" for splicing that will enable prediction of the splicing pattern of any primary transcript from its sequence. Outside of the core splice site motifs, the bulk of the information required for splicing is thought to be contained in exonic and intronic cis-regulatory elements that function by recruitment of sequence-specific RNA-binding protein factors that either activate or repress the use of adjacent splice sites. Here, we summarize the current state of knowledge of splicing cis-regulatory elements and their context-dependent effects on splicing, emphasizing recent global/genome-wide studies and open questions.

MeSH Terms
Alternative Splicing Animals Enhancer Elements, Genetic Exons Gene Expression Regulation Genes, Regulator Humans Introns Models, Genetic RNA Precursors/genetics RNA Splicing Silencer Elements, Transcriptional
Chemicals
RNA Precursors
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wang Zefeng
Department of Pharmacology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. zefeng@med.unc.edu
Burge Christopher B
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Article Info
Journal
RNA (New York, N.Y.)
Abbr.
RNA
ISSN
1469-9001
DOI
10.1261/rna.876308
Email
zefeng@med.unc.edu
Published
2008-05-00
Epub
2008-00-27
Pages
802-13
Language
English
Region
United States
NLM ID
9509184
PMCID
PMC2327353
Subset
IM
External Links
PubMed source DOI: 10.1261/rna.876308 PMC Full Text View journal details
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