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PMID: 18364464 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Haplo-insufficiency of MPK3 in MPK6 mutant background uncovers a novel function of these two MAPKs in Arabidopsis ovule development.

The Plant cell ·Vol. 20 ·No. 3 ·2008-03-00 ·Pages 602-13

Wang H, Liu Y, Bruffett K, Lee J, Hause G, Walker JC, Zhang S

Abstract

The plant life cycle includes diploid sporophytic and haploid gametophytic generations. Female gametophytes (embryo sacs) in higher plants are embedded in specialized sporophytic structures (ovules). Here, we report that two closely related mitogen-activated protein kinases in Arabidopsis thaliana, MPK3 and MPK6, share a novel function in ovule development: in the MPK6 mutant background, MPK3 is haplo-insufficient, giving female sterility when heterozygous. By contrast, in the MPK3 mutant background, MPK6 does not show haplo-insufficiency. Using wounding treatment, we discovered gene dosage-dependent activation of MPK3 and MPK6. In addition, MPK6 activation is enhanced when MPK3 is null, which may help explain why mpk3(-/-) mpk6(+/-) plants are fertile. Genetic analysis revealed that the female sterility of mpk3(+/-) mpk6(-/-) plants is a sporophytic effect. In mpk3(+/-) mpk6(-/-) mutant plants, megasporogenesis and megagametogenesis are normal and the female gametophyte identity is correctly established. Further analysis demonstrates that the mpk3(+/-) mpk6(-/-) ovules have abnormal integument development with arrested cell divisions at later stages. The mutant integuments fail to accommodate the developing embryo sac, resulting in the embryo sacs being physically restricted and female reproductive failure. Our results highlight an essential function of MPK3 and MPK6 in promoting cell division in the integument specifically during ovule development.

MeSH Terms
Arabidopsis/genetics,growth & development,ultrastructure Arabidopsis Proteins/genetics,metabolism Flowers/genetics,growth & development,ultrastructure Gene Expression Regulation, Developmental Gene Expression Regulation, Plant Microscopy, Electron, Scanning Mitogen-Activated Protein Kinase Kinases/genetics,metabolism Mitogen-Activated Protein Kinases/genetics,metabolism Mutation Plant Infertility/genetics Plants, Genetically Modified
Chemicals
Arabidopsis Proteins AtMPK3 protein, Arabidopsis MPK6 protein, Arabidopsis Mitogen-Activated Protein Kinases Mitogen-Activated Protein Kinase Kinases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Wang Huachun
Department of Biochemistry and Bond Life Sciences Center, University of Missouri, Columbia, Missouri 65211, USA.
Liu Yidong
Bruffett Kristin
Lee Justin
Hause Gerd
Walker John C
Zhang Shuqun
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Article Info
Journal
The Plant cell
Abbr.
Plant Cell
ISSN
1040-4651
DOI
10.1105/tpc.108.058032
Published
2008-03-00
Epub
2008-00-25
Pages
602-13
Language
English
Region
England
NLM ID
9208688
PMCID
PMC2329925
Subset
IM
External Links
PubMed source DOI: 10.1105/tpc.108.058032 PMC Full Text View journal details
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