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PMID: 1836010 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

T cell receptor genes in a series of class I major histocompatibility complex-restricted cytotoxic T lymphocyte clones specific for a Plasmodium berghei nonapeptide: implications for T cell allelic exclusion and antigen-specific repertoire.

The Journal of experimental medicine ·Vol. 174 ·No. 6 ·1991-12-01 ·Pages 1371-83

Casanova JL, Romero P, Widmann C, Kourilsky P, Maryanski JL

Abstract

We report here the first extensive study of a T cell repertoire for a class I major histocompatibility complex (MHC)-restricted cytotoxic T lymphocyte (CTL) response. We have found that the T cell receptors (TCRs) carried by 28 H-2Kd-restricted CTL clones specific for a single Plasmodium berghei circumsporozoite nonapeptide are highly diverse in terms of V alpha, J alpha, and J beta segments and aminoacid composition of the junctional regions. However, despite this extensive diversity, a high proportion of the TCRs contain the same V beta segment. These results are in contrast to most previously reported T cell responses towards class II MHC-peptide complexes, where the TCR repertoires appeared to be much more limited. In our study, the finding of a dominant V beta in the midst of otherwise highly diverse TCRs suggests the importance of the V beta segment in shaping the T cell repertoire specific for a given MHC-peptide complex. As an additional finding, we observed that nearly all clones have rearranged both TCR alpha loci. Moreover, as many as one-third of the CTL clones that we analyzed apparently display two productive alpha rearrangements. This argues against a regulated model of sequential recombination at the alpha locus and consequently raises the question of whether allelic exclusion of the TCR alpha chain is achieved at all.

MeSH Terms
Alleles Amino Acid Sequence Animals Base Sequence Clone Cells Gene Rearrangement, T-Lymphocyte H-2 Antigens/immunology Mice Mice, Inbred BALB C Molecular Sequence Data Plasmodium berghei/immunology Protozoan Proteins/immunology Receptors, Antigen, T-Cell, alpha-beta/chemistry,genetics T-Lymphocytes, Cytotoxic/immunology Transcription, Genetic
Chemicals
H-2 Antigens Protozoan Proteins Receptors, Antigen, T-Cell, alpha-beta
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Casanova J L
Biologie Moléculaire du Gène, INSERM U277, Institut Pasteur, Paris.
Romero P
Widmann C
Kourilsky P
Maryanski J L
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.174.6.1371
Published
1991-12-01
Pages
1371-83
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2119045
Subset
IM
Databases
GENBANK
X60837, X60838, X60839, X60840, X60841, X60842, X60843, X60844, X60845, X60846, X60847, X60848, X60849, X60850, X60851, X60852, X60853, X60854, X60855, X60856, X60857, X60858, X60859, X60860, X60861, X60862, X60863, X60864, X60865, X60866
External Links
PubMed source DOI: 10.1084/jem.174.6.1371 PMC Full Text View journal details
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