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PMID: 18353776 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

cAMP inhibits cell migration by interfering with Rac-induced lamellipodium formation.

The Journal of biological chemistry ·Vol. 283 ·No. 20 ·2008-05-16 ·Pages 13799-805

Chen L, Zhang JJ, Huang XY

Abstract

Cell migration is critical for animal development and physiological as well as pathological responses. One important step during cell migration is the formation of lamellipodia at the leading edge of migrating cells. Here we report that the second messenger cAMP inhibits the migration of mouse embryonic fibroblast cells and mouse breast tumor cells. cAMP acts downstream of the small GTPase Rac and interferes with the formation of lamellipodia. Moreover, cAMP decreases the phosphorylation of the myosin light chain at the leading edge of cells and increases the phosphorylation of the vasodilator-stimulated phosphoprotein. Together with our previous report of a positive role of another second messenger, cGMP, in lamellipodium formation, our data indicate that cAMP and cGMP play opposite roles in modulating lamellipodium formation.

MeSH Terms
1-Methyl-3-isobutylxanthine/pharmacology Animals Breast Neoplasms/metabolism Cell Line, Tumor Cell Movement Cyclic AMP/metabolism Fibroblasts/metabolism Humans Mice Mice, Transgenic Models, Biological Myosin Light Chains/metabolism Phosphorylation Pseudopodia/metabolism rac GTP-Binding Proteins/metabolism
Chemicals
Myosin Light Chains Cyclic AMP rac GTP-Binding Proteins 1-Methyl-3-isobutylxanthine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Chen Lin
Department of Physiology, Cornell University Weill Medical College, New York, New York 10021, USA.
Zhang J Jillian
Huang Xin-Yun
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Article Info
Journal
The Journal of biological chemistry
Abbr.
J Biol Chem
ISSN
0021-9258
DOI
10.1074/jbc.M800555200
Published
2008-05-16
Epub
2008-00-19
Pages
13799-805
Language
English
Region
United States
NLM ID
2985121R
PMCID
PMC2376213
Subset
IM
Grants
NIA NIH HHS · AG23202 · United States
External Links
PubMed source DOI: 10.1074/jbc.M800555200 PMC Full Text View journal details
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