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PMID: 18338249 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Transcription factor GATA-3 regulates the transcriptional activity of dopamine beta-hydroxylase by interacting with Sp1 and AP4.

Neurochemical research ·Vol. 33 ·No. 9 ·2008-09-00 ·Pages 1821-31

Hong SJ, Choi HJ, Hong S, Huh Y, Chae H, Kim KS

Abstract

GATA-3 is a zinc finger transcription factor that is expressed in T cell lineages as well as in the nervous system during development. In this study, we report that forced expression of GATA-3 resulted in an increased number of dopamine beta-hydroxylase (DBH)-expressing neurons in primary neural crest stem cell (NCSC) culture, suggesting that the DBH gene may be a downstream target gene of GATA-3. GATA-3 robustly transactivates the promoter function of the noradrenaline (NA)-synthesizing DBH gene, via two specific upstream promoter domains; one at -62 to -32 bp and the other at -891 to -853 bp. Surprisingly, none of these domains contain GATA-3 binding sites but encompass binding motifs for transcription factors Sp1 and AP4, respectively. Protein-protein interaction analyses both in vitro and in vivo and chromatin immunoprecipitation (ChIP) assays showed that GATA-3 effects its transcriptional regulatory function through physical interactions with these transcription factors.

MeSH Terms
Animals Cells, Cultured Dopamine beta-Hydroxylase/genetics,metabolism GATA3 Transcription Factor/genetics,metabolism Gene Expression Regulation Humans Neurons/cytology,physiology Promoter Regions, Genetic Quail Recombinant Fusion Proteins/genetics,metabolism Sp1 Transcription Factor/genetics,metabolism Transcription Factors/genetics,metabolism Transcription, Genetic
Chemicals
GATA3 Transcription Factor Recombinant Fusion Proteins Sp1 Transcription Factor Transcription Factors Dopamine beta-Hydroxylase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Hong Seok Jong
Molecular Neurobiology Laboratory, MRC215, McLean Hospital, Harvard Medical School, 115 Mill Street, Belmont, MA, 02478, USA.
Choi Hyun Jin
Hong Sunghoi
Huh Youngbuhm
Chae Han
Kim Kwang-Soo
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Article Info
Journal
Neurochemical research
Abbr.
Neurochem Res
ISSN
1573-6903
DOI
10.1007/s11064-008-9639-3
Published
2008-09-00
Epub
2008-00-13
Pages
1821-31
Language
English
Region
United States
NLM ID
7613461
PMCID
PMC2712938
Subset
IM
Grants
NIMH NIH HHS · R01 MH048866 · United States
NIMH NIH HHS · R01 MH048866-17 · United States
NINDS NIH HHS · R01 NS084869 · United States
NIMH NIH HHS · R29 MH048866 · United States
NIDCD NIH HHS · R01 DC006501 · United States
NIMH NIH HHS · MH48866 · United States
NIDCD NIH HHS · DC006501 · United States
NIDCD NIH HHS · R01 DC006501-05 · United States
External Links
PubMed source DOI: 10.1007/s11064-008-9639-3 PMC Full Text View journal details
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