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PMID: 1833067 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

An evolutionarily conserved cyclin homolog from Drosophila rescues yeast deficient in G1 cyclins.

Cell ·Vol. 66 ·No. 6 ·1991-09-20 ·Pages 1207-16

Léopold P, O'Farrell PH

Abstract

We have isolated two Drosophila cDNA clones that rescue Saccharomyces cerevisiae deficient in CLN functions. One of these clones is the Drosophila homolog of the cdc2 gene. The second encodes a distant and new member of the cyclin family of proteins, cyclin C. It is highly homologous (72% identity) to a human clone isolated in a similar screen. Yeast cells rescued by a plasmid constitutively expressing this Drosophila cyclin C are unusually small, consistent with an unregulated high level of G1 cyclin function. Sequence comparisons identified regions conserved among the more distantly related cyclins. Based on these conserved elements, we identified homology between cyclins and the ras oncogene.

Related Genes
Cln1 Cln3 cdc13 cycA cycB prad1
MeSH Terms
Amino Acid Sequence Animals Base Sequence Blotting, Northern CDC2 Protein Kinase/physiology Cell Cycle Cloning, Molecular Cyclins/classification,genetics DNA/genetics Drosophila melanogaster/genetics Gene Expression Genetic Complementation Test Molecular Sequence Data RNA, Messenger/genetics Saccharomyces cerevisiae/genetics Sequence Alignment Species Specificity
Chemicals
Cyclins RNA, Messenger DNA CDC2 Protein Kinase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Léopold P
Department of Biochemistry and Biophysics, University of California, San Francisco 94143.
O'Farrell P H
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45 references, click to expand
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Article Info
Journal
Cell
Abbr.
Cell
ISSN
0092-8674
DOI
10.1016/0092-8674(91)90043-x
Published
1991-09-20
Pages
1207-16
Language
English
Region
United States
NLM ID
0413066
PMCID
PMC2753436
Subset
IM
Grants
NIGMS NIH HHS · R01 GM037193 · United States
NIGMS NIH HHS · R01 GM037193-10 · United States
NHLBI NIH HHS · P01 HL43821 · United States
Databases
GENBANK
M74906
External Links
PubMed source DOI: 10.1016/0092-8674(91)90043-x PMC Full Text View journal details
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