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PMID: 1831458 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Evolutionary conservation of a microbody targeting signal that targets proteins to peroxisomes, glyoxysomes, and glycosomes.

The Journal of cell biology ·Vol. 114 ·No. 5 ·1991-09-00 ·Pages 893-904

Keller GA, Krisans S, Gould SJ, Sommer JM, Wang CC, Schliebs W, Kunau W, Brody S, Subramani S

Abstract

Peroxisomes, glyoxysomes, glycosomes, and hydrogenosomes have each been classified as microbodies, i.e., subcellular organelles with an electron-dense matrix that is bound by a single membrane. We investigated whether these organelles might share a common evolutionary origin by asking if targeting signals used for translocation of proteins into these microbodies are related. A peroxisomal targeting signal (PTS) consisting of the COOH-terminal tripeptide serine-lysine-leucine-COOH has been identified in a number of peroxisomal proteins (Gould, S.J., G.-A. Keller, N. Hosken, J. Wilkinson, and S. Subramani. 1989. J. Cell Biol. 108:1657-1664). Antibodies raised to a peptide ending in this sequence (SKL-COOH) recognize a number of peroxisomal proteins. Immunocryoelectron microscopy experiments using this anti-SKL antibody revealed the presence of proteins containing the PTS within glyoxysomes of cells from Pichia pastoris, germinating castor bean seeds, and Neurospora crassa, as well as within the glycosomes of Trypanosoma brucei. Western blot analysis of purified organelle fractions revealed the presence of many proteins containing this PTS in both glyoxysomes and glycosomes. These results indicate that at least one of the signals, and therefore the mechanism, for protein translocation into peroxisomes, glyoxysomes, and glycosomes has been conserved, lending support to a common evolutionary origin for these microbodies. Hydrogenosomes, the fourth type of microbody, did not contain proteins that cross-reacted with the anti-PTS antibody, suggesting that this organelle is unrelated to microbodies.

MeSH Terms
Amino Acid Sequence Animals Biological Evolution Biological Transport Blotting, Western Cell Compartmentation Microbodies/metabolism Microscopy, Electron Molecular Sequence Data Molecular Weight Neurospora crassa Organelles/metabolism Peptides/chemistry Plants, Toxic Proteins/metabolism Ricinus Trypanosoma brucei brucei/analysis Yeasts
Chemicals
Peptides Proteins
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Keller G A
Department of Preclinical Pharmacology, Genentech Inc., South San Francisco, California 94080.
Krisans S
Gould S J
Sommer J M
Wang C C
Schliebs W
Kunau W
Brody S
Subramani S
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.114.5.893
Published
1991-09-00
Pages
893-904
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2289121
Subset
IM
Grants
NIDDK NIH HHS · DK41737 · United States
External Links
PubMed source DOI: 10.1083/jcb.114.5.893 PMC Full Text View journal details
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