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PMID: 18296522 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

DivIVA is required for polar growth in the MreB-lacking rod-shaped actinomycete Corynebacterium glutamicum.

Journal of bacteriology ·Vol. 190 ·No. 9 ·2008-05-00 ·Pages 3283-92

Letek M, Ordóñez E, Vaquera J, Margolin W, Flärdh K, Mateos LM, Gil JA

Abstract

The actinomycete Corynebacterium glutamicum grows as rod-shaped cells by zonal peptidoglycan synthesis at the cell poles. In this bacterium, experimental depletion of the polar DivIVA protein (DivIVA(Cg)) resulted in the inhibition of polar growth; consequently, these cells exhibited a coccoid morphology. This result demonstrated that DivIVA is required for cell elongation and the acquisition of a rod shape. DivIVA from Streptomyces or Mycobacterium localized to the cell poles of DivIVA(Cg)-depleted C. glutamicum and restored polar peptidoglycan synthesis, in contrast to DivIVA proteins from Bacillus subtilis or Streptococcus pneumoniae, which localized at the septum of C. glutamicum. This confirmed that DivIVAs from actinomycetes are involved in polarized cell growth. DivIVA(Cg) localized at the septum after cell wall synthesis had started and the nucleoids had already segregated, suggesting that in C. glutamicum DivIVA is not involved in cell division or chromosome segregation.

MeSH Terms
Bacterial Proteins/analysis,genetics,metabolism Cell Cycle Proteins/analysis,genetics,metabolism Cell Division/genetics Cell Enlargement Cell Polarity/genetics Corynebacterium glutamicum/cytology,genetics,growth & development Genetic Complementation Test
Chemicals
Bacterial Proteins Cell Cycle Proteins DivIVA protein, bacteria
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Letek Michal
Departamento de Biología Molecular, Area de Microbiología, Facultad de Biología, Universidad de León, 24071 León, Spain.
Ordóñez Efrén
Vaquera José
Margolin William
Flärdh Klas
Mateos Luis M
Gil José A
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
1098-5530
DOI
10.1128/JB.01934-07
Published
2008-05-00
Epub
2008-00-22
Pages
3283-92
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC2347398
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.01934-07 PMC Full Text View journal details
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