Home LiteratureArticle Details
PMID: 1829455 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Biosynthesis of the Escherichia coli K5 polysaccharide, a representative of group II capsular polysaccharides: polymerization in vitro and characterization of the product.

Journal of bacteriology ·Vol. 173 ·No. 13 ·1991-07-00 ·Pages 4088-94

Finke A, Bronner D, Nikolaev AV, Jann B, Jann K

Abstract

Biosynthesis of the capsular K5 polysaccharide of Escherichia coli, which has the structure 4)-beta GlcA-1,4-alpha GlcNAc-(1, was studied with membrane preparations from an E. coli K5 wild-type strain and from a recombinant K-12 strain expressing the K5 capsule. Polymerization occurs at the inner face of the cytoplasmic membrane without the participation of lipid-linked oligosaccharides. The serological K5 specificity of the in vitro product was determined with a K5-specific monoclonal antibody in an antigen-binding assay. The K5 polysaccharide, as obtained from the membranes after an in vitro incubation, has 2-keto-3-deoxyoctulosonic acid as the reducing sugar, which indicates that the polysaccharide grows by chain elongation at the nonreducing end.

MeSH Terms
Antigens, Bacterial/biosynthesis Bacterial Capsules Cell Membrane/enzymology,metabolism Escherichia coli/drug effects,enzymology,metabolism Glucosyltransferases/metabolism Kinetics Macromolecular Substances Polysaccharides, Bacterial/biosynthesis,chemistry,physiology Sugar Acids/metabolism Tunicamycin/pharmacology
Chemicals
Antigens, Bacterial Macromolecular Substances Polysaccharides, Bacterial Sugar Acids 2-keto-3-deoxyoctonate Tunicamycin capsular polysaccharide K5 Glucosyltransferases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Finke A
Max-Planck-Institut für Immunobiologie, Freiburg, Federal Republic of Germany.
Bronner D
Nikolaev A V
Jann B
Jann K
References (38)
38 references, click to expand
  1. Biosynthesis and assembly of the polysialic acid capsule in Escherichia coli K1. Role of a low-density vesicle fraction in activation of the endogenous synthesis of sialyl polymers.
    J Biol Chem. 1984 Oct 25;259(20):12769-75 PMID: 6386802
  2. Biosynthesis and assembly of the polysialic acid capsule in Escherichia coli K1. Activation of sialyl polymer synthesis in inactivate sialyltransferase complexes requires protein synthesis.
    J Biol Chem. 1984 Oct 25;259(20):12776-80 PMID: 6386803
  3. Biosynthesis of the polysialic acid capsule in Escherichia coli K1. The endogenous acceptor of polysialic acid is a membrane protein of 20 kDa.
    J Biol Chem. 1990 Jan 25;265(3):1578-87 PMID: 2404016
  4. CMP-KDO-synthetase activity in Escherichia coli expressing capsular polysaccharides.
    FEMS Microbiol Lett. 1990 May;57(1-2):129-33 PMID: 2165964
  5. Isolation and characterization of two outer membrane preparations from Escherichia coli.
    Biochim Biophys Acta. 1975 Jan 14;375(1):44-53 PMID: 1089432
  6. Cell-wall lipopolysaccharides of ampicillin-resistant mutants of Escherichia coli K-12.
    Eur J Biochem. 1976 JUL 1;66(2):369-77 PMID: 780112
  7. Genetics of capsular polysaccharide production in bacteria.
    Curr Top Microbiol Immunol. 1990;150:1-18 PMID: 2105187
  8. Further electron microscopic studies on the expression of Escherichia coli group II capsules.
    J Bacteriol. 1990 Jun;172(6):3469-72 PMID: 2188961
  9. Structure and biosynthesis of the capsular antigens of Escherichia coli.
    Curr Top Microbiol Immunol. 1990;150:19-42 PMID: 2404688
  10. Expression of the Escherichia coli K5 capsular antigen: immunoelectron microscopic and biochemical studies with recombinant E. coli.
    J Bacteriol. 1990 Feb;172(2):1085-91 PMID: 2404935
  11. The structure of the capsular polysaccharide (K5 antigen) of urinary-tract-infective Escherichia coli 010:K5:H4. A polymer similar to desulfo-heparin.
    Eur J Biochem. 1981 May 15;116(2):359-64 PMID: 7018909
  12. Genetic and molecular analyses of Escherichia coli K1 antigen genes.
    J Bacteriol. 1984 Feb;157(2):568-75 PMID: 6319367
  13. Assay of N-acetylheparosan deacetylase with a capsular polysaccharide from Escherichia coli K5 as substrate.
    Anal Biochem. 1983 Nov;135(1):134-40 PMID: 6367539
  14. Protein synthesis is required for in vivo activation of polysialic acid capsule synthesis in Escherichia coli K1.
    J Bacteriol. 1984 Jul;159(1):321-8 PMID: 6376473
  15. Cell-free biosynthesis of the O-acetylated N-acetylneuraminic acid capsular polysaccharide of group C meningococci.
    J Bacteriol. 1978 Mar;133(3):1300-6 PMID: 25263
  16. Role of a membranous sialyltransferase complex in the synthesis of surface polymers containing polysialic acid in Escherichia coli. Temperature-induced alteration in the assembly process.
    J Biol Chem. 1979 Aug 10;254(15):7377-87 PMID: 379003
  17. Biosynthesis of the O9 antigen of Escherichia coli. Growth of the polysaccharide chain.
    Eur J Biochem. 1978 Feb 1;83(1):47-52 PMID: 342245
  18. Serology, chemistry, and genetics of O and K antigens of Escherichia coli.
    Bacteriol Rev. 1977 Sep;41(3):667-710 PMID: 334154
  19. Biological activities of the two major components of tunicamycin.
    J Biol Chem. 1979 Jul 25;254(14):6572-6 PMID: 447736
  20. Enzyme-linked immunosorbent assay, Elisa. 3. Quantitation of specific antibodies by enzyme-labeled anti-immunoglobulin in antigen-coated tubes.
    J Immunol. 1972 Jul;109(1):129-35 PMID: 4113792
  21. Genetic mapping of the antigenic determinants of two polysaccharide K antigens, K10 and K54, in Escherichia coli.
    J Bacteriol. 1974 Oct;120(1):43-51 PMID: 4138850
  22. Mechanism of assembly of the outer membrane of Salmonella typhimurium. Isolation and characterization of cytoplasmic and outer membrane.
    J Biol Chem. 1972 Jun 25;247(12):3962-72 PMID: 4555955
  23. Tunicamycin inhibition of polyisoprenyl N-acetylglucosaminyl pyrophosphate formation in calf-liver microsomes.
    Biochem Biophys Res Commun. 1975 Jul 8;65(1):248-57 PMID: 167767
  24. Role of undecaprenyl phosphate in synthesis of polymers containing sialic acid in Escherichia coli.
    J Biol Chem. 1975 Jan 10;250(1):156-63 PMID: 1095566
  25. Bacitracin: an inhibitor of the dephosphorylation of lipid pyrophosphate, an intermediate in the biosynthesis of the peptidoglycan of bacterial cell walls.
    Proc Natl Acad Sci U S A. 1967 Mar;57(3):767-73 PMID: 16591529
  26. Structure and serological characteristics of the capsular K4 antigen of Escherichia coli O5:K4:H4, a fructose-containing polysaccharide with a chondroitin backbone.
    Eur J Biochem. 1988 Oct 15;177(1):117-24 PMID: 2460347
  27. Proton-conducting portion (F0) from Escherichia coli ATP synthase: preparation, dissociation into subunits, and reconstitution of an active complex.
    Methods Enzymol. 1986;126:569-78 PMID: 2908466
  28. Common organization of gene clusters for production of different capsular polysaccharides (K antigens) in Escherichia coli.
    J Bacteriol. 1988 Mar;170(3):1305-10 PMID: 2830235
  29. A protein-sialyl polymer complex involved in colominic acid biosynthesis. Effect of tunicamycin.
    Biochem J. 1988 Apr 15;251(2):589-96 PMID: 3041966
  30. Activity of CMP-2-keto-3-deoxyoctulosonic acid synthetase in Escherichia coli strains expressing the capsular K5 polysaccharide implication for K5 polysaccharide biosynthesis.
    J Bacteriol. 1989 Jun;171(6):3074-9 PMID: 2542215
  31. Characterization of an Escherichia coli rff mutant defective in transfer of N-acetylmannosaminuronic acid (ManNAcA) from UDP-ManNAcA to a lipid-linked intermediate involved in enterobacterial common antigen synthesis.
    J Bacteriol. 1988 Jan;170(1):228-33 PMID: 3275612
  32. Role of lipid intermediate(s) in the synthesis of serogroup B Neisseria meningitidis capsular polysaccharide.
    J Bacteriol. 1985 Mar;161(3):861-7 PMID: 3918990
  33. Polyprenyl phosphates: synthesis and structure-activity relationship for a biosynthetic system of Salmonella anatum O-specific polysaccharide.
    Chem Phys Lipids. 1989 Nov;51(3-4):191-203 PMID: 2611960
  34. Molecular cloning and analysis of genes for production of K5, K7, K12, and K92 capsular polysaccharides in Escherichia coli.
    J Bacteriol. 1986 Dec;168(3):1228-33 PMID: 3023300
  35. Biosynthesis of enterobacterial common antigen in Escherichia coli. In vitro synthesis of lipid-linked intermediates.
    J Biol Chem. 1987 May 25;262(15):7142-50 PMID: 3034883
  36. Biosynthesis of the O9 antigen of Escherichia coli. Synthetic glycosyldiphosphomoraprenols as probes for requirement of mannose acceptors.
    Eur J Biochem. 1985 Sep 2;151(2):393-7 PMID: 2411556
  37. Bacterial polysaccharide capsule synthesis, export and evolution of structural diversity.
    Mol Microbiol. 1989 Dec;3(12):1819-23 PMID: 2695750
  38. Influence of growth temperature on the development of Escherichia coli polysaccharide K antigens.
    J Gen Microbiol. 1984 Oct;130(10):2681-4 PMID: 6392476
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.173.13.4088-4094.1991
Published
1991-07-00
Pages
4088-94
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC208057
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.173.13.4088-4094.1991 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2