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PMID: 18287041 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Arabidopsis LEAFY COTYLEDON2 induces maturation traits and auxin activity: Implications for somatic embryogenesis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 105 ·No. 8 ·2008-02-26 ·Pages 3151-6

Stone SL, Braybrook SA, Paula SL, Kwong LW, Meuser J, Pelletier J, Hsieh TF, Fischer RL, Goldberg RB, Harada JJ

Abstract

LEAFY COTYLEDON2 (LEC2) is a central regulator of embryogenesis sufficient to induce somatic cells to form embryos when expressed ectopically. Here, we analyze the cellular processes induced by LEC2, a B3 domain transcription factor, that may underlie its ability to promote somatic embryogenesis. We show auxin-responsive genes are induced after LEC2 activation in seedlings. Genes encoding enzymes involved in auxin biosynthesis, YUC2 and YUC4, are activated within 1 h after induction of LEC2 activity, and YUC4 appears to be a direct transcriptional target of LEC2. We also show ectopic LEC2 expression induces accumulation of seed storage protein and oil bodies in vegetative and reproductive organs, events that normally occur during the maturation phase of embryogenesis. Furthermore, LEC2 activates seed protein genes before an increase in RNAs encoding LEC1 or FUS3 is observed. Thus, LEC2 causes rapid changes in auxin responses and induces cellular differentiation characteristic of the maturation phase. The relevance of these changes to the ability of LEC2 to promote somatic embryogenesis is discussed.

MeSH Terms
Arabidopsis/embryology,genetics Arabidopsis Proteins/physiology Cell Differentiation/genetics Chromatin Immunoprecipitation DNA Primers/genetics Embryonic Development/genetics Gene Expression Regulation, Developmental/genetics Indoleacetic Acids/metabolism Reverse Transcriptase Polymerase Chain Reaction Seeds/metabolism,physiology Transcription Factors/physiology
Chemicals
Arabidopsis Proteins DNA Primers Indoleacetic Acids LEC2 protein, Arabidopsis Transcription Factors
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Stone Sandra L
Section of Plant Biology, College of Biological Sciences, and Graduate Program in Plant Biology, University of California, Davis, CA 95616, USA.
Braybrook Siobhan A
Paula Stephanie L
Kwong Linda W
Meuser Jonathan
Pelletier Julie
Hsieh Tzung-Fu
Fischer Robert L
Goldberg Robert B
Harada John J
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
1091-6490
DOI
10.1073/pnas.0712364105
Published
2008-02-26
Epub
2008-00-19
Pages
3151-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC2268600
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.0712364105 PMC Full Text View journal details
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