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PMID: 18283488 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Characterization of PsMPK2, the first C1 subgroup MAP kinase from pea (Pisum sativum L.).

Planta ·Vol. 227 ·No. 6 ·2008-05-00 ·Pages 1333-42

Ortiz-Masia D, Perez-Amador MA, Carbonell P, Aniento F, Carbonell J, Marcote MJ

Abstract

Mitogen-activated protein kinase (MAPK) cascades play a key role in plant growth and development as well as in biotic and abiotic stress responses. They are classified according to their sequence homology into four major groups (A-D). A large amount of information about MAPKs in groups A and B is available but few data of the C group have been reported. In this study, a C1 subgroup MAP kinase cDNA, PsMPK2, was isolated from Pisum sativum. PsMPK2 is expressed in vegetative (root and leaf) and reproductive (stamen, pistil and fruit) organs. Expression of PsMPK2 in Arabidopsis thaliana shows that mechanical injury and other stress signals as abscisic acid, jasmonic acid and hydrogen peroxide increase its kinase activity, extending previous results indicating that C1 subgroup MAPKs may be involved in the response to stress.

MeSH Terms
Amino Acid Sequence DNA, Complementary/genetics DNA, Plant/genetics Kinetics Mitogen-Activated Protein Kinases/genetics,isolation & purification,metabolism Molecular Sequence Data Peas/enzymology Plant Proteins/genetics,isolation & purification,metabolism Restriction Mapping Reverse Transcriptase Polymerase Chain Reaction
Chemicals
DNA, Complementary DNA, Plant Plant Proteins Mitogen-Activated Protein Kinases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Ortiz-Masia Dolores
Facultad de Farmacia, Universidad de Valencia, Avda. Vicente Andrés Estellés s/n, 46100, Burjassot, Spain.
Perez-Amador Miguel A
Carbonell Pablo
Aniento Fernando
Carbonell Juan
Marcote Maria J
References (35)
35 references, click to expand
  1. The Pisum sativum MAP kinase homologue (PsMAPK) rescues the Saccharomyces cerevisiae hog1 deletion mutant under conditions of high osmotic stress.
    Plant Mol Biol. 1996 May;31(2):355-63 PMID: 8756598
  2. Cyclin D1 is a nuclear protein required for cell cycle progression in G1.
    Genes Dev. 1993 May;7(5):812-21 PMID: 8491378
  3. BAS1: A gene regulating brassinosteroid levels and light responsiveness in Arabidopsis.
    Proc Natl Acad Sci U S A. 1999 Dec 21;96(26):15316-23 PMID: 10611382
  4. Arabidopsis kinome: after the casting.
    Funct Integr Genomics. 2004 Jul;4(3):163-87 PMID: 14740254
  5. A Mitogen-activated protein kinase kinase kinase mediates reactive oxygen species homeostasis in Arabidopsis.
    J Biol Chem. 2006 Dec 15;281(50):38697-704 PMID: 17043356
  6. Isolation and characterization of a tobacco cDNA clone encoding a putative MAP kinase.
    Plant Mol Biol. 1993 Nov;23(3):543-51 PMID: 8219089
  7. Mutational evidence that the Arabidopsis MAP kinase MPK6 is involved in anther, inflorescence, and embryo development.
    J Exp Bot. 2007;58(8):2181-91 PMID: 17519351
  8. The PHD domain of MEKK1 acts as an E3 ubiquitin ligase and mediates ubiquitination and degradation of ERK1/2.
    Mol Cell. 2002 May;9(5):945-56 PMID: 12049732
  9. GENEVESTIGATOR. Arabidopsis microarray database and analysis toolbox.
    Plant Physiol. 2004 Sep;136(1):2621-32 PMID: 15375207
  10. Stomatal development and patterning are regulated by environmentally responsive mitogen-activated protein kinases in Arabidopsis.
    Plant Cell. 2007 Jan;19(1):63-73 PMID: 17259259
  11. The MKK2 pathway mediates cold and salt stress signaling in Arabidopsis.
    Mol Cell. 2004 Jul 2;15(1):141-52 PMID: 15225555
  12. Diverse signals converge at MAPK cascades in plant.
    Plant Physiol Biochem. 2006 May-Jun;44(5-6):274-83 PMID: 16809044
  13. Various abiotic stresses rapidly activate Arabidopsis MAP kinases ATMPK4 and ATMPK6.
    Plant J. 2000 Dec;24(5):655-65 PMID: 11123804
  14. Distinct regulation of salinity and genotoxic stress responses by Arabidopsis MAP kinase phosphatase 1.
    EMBO J. 2002 Dec 2;21(23):6483-93 PMID: 12456655
  15. The Arabidopsis mitogen-activated protein kinase kinase MKK3 is upstream of group C mitogen-activated protein kinases and participates in pathogen signaling.
    Plant Cell. 2007 Oct;19(10):3266-79 PMID: 17933903
  16. The mitogen-activated protein kinase cascade MKK3-MPK6 is an important part of the jasmonate signal transduction pathway in Arabidopsis.
    Plant Cell. 2007 Mar;19(3):805-18 PMID: 17369371
  17. Molecular cloning and expression of a MAP kinase homologue from pea.
    Plant Mol Biol. 1993 Apr;22(1):83-90 PMID: 8388749
  18. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  19. Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana.
    Plant J. 1998 Dec;16(6):735-43 PMID: 10069079
  20. RNA interference-based (RNAi) suppression of AtMPK6, an Arabidopsis mitogen-activated protein kinase, results in hypersensitivity to ozone and misregulation of AtMPK3.
    Environ Pollut. 2005 Nov;138(2):230-7 PMID: 15964670
  21. Silencing of the mitogen-activated protein kinase MPK6 compromises disease resistance in Arabidopsis.
    Plant Cell. 2004 Apr;16(4):897-907 PMID: 15020743
  22. MEKK1 is required for flg22-induced MPK4 activation in Arabidopsis plants.
    Plant Physiol. 2007 Feb;143(2):661-9 PMID: 17142480
  23. Ribulose-1,5-bisphosphate carboxylase and fruit set or degeneration of unpollinated ovaries of Pisum sativum L.
    Planta. 1985 Jul;164(4):534-9 PMID: 24248229
  24. Mitogen-activated protein kinase cascades in plants: a new nomenclature.
    Trends Plant Sci. 2002 Jul;7(7):301-8 PMID: 12119167
  25. Arabidopsis map kinase 4 negatively regulates systemic acquired resistance.
    Cell. 2000 Dec 22;103(7):1111-20 PMID: 11163186
  26. A homologue of the MAP/ERK family of protein kinase genes is expressed in vegetative and in female reproductive organs of Petunia hybrida.
    Plant Mol Biol. 1995 Jan;27(2):339-50 PMID: 7888623
  27. Diverse stress signals activate the C1 subgroup MAP kinases of Arabidopsis.
    FEBS Lett. 2007 May 1;581(9):1834-40 PMID: 17433310
  28. Signaling through MAP kinase networks in plants.
    Arch Biochem Biophys. 2006 Aug 1;452(1):55-68 PMID: 16806044
  29. ATMPKs: a gene family of plant MAP kinases in Arabidopsis thaliana.
    FEBS Lett. 1993 Dec 28;336(3):440-4 PMID: 8282107
  30. Emerging MAP kinase pathways in plant stress signalling.
    Trends Plant Sci. 2005 Jul;10(7):339-46 PMID: 15953753
  31. The Arabidopsis MAP kinase kinase MKK1 participates in defence responses to the bacterial elicitor flagellin.
    Plant J. 2006 Nov;48(4):485-98 PMID: 17059410
  32. Transient expression of a pea MAP kinase gene induced by gibberellic acid and 6-benzyladenine in unpollinated pea ovaries.
    Plant Mol Biol. 2000 Sep;44(2):177-86 PMID: 11117261
  33. MAP kinase signalling cascade in Arabidopsis innate immunity.
    Nature. 2002 Feb 28;415(6875):977-83 PMID: 11875555
  34. Soluble expression and characterization of a GFP-fused pea actin isoform (PEAc1).
    Cell Res. 2004 Oct;14(5):407-14 PMID: 15538972
  35. MEKK1 is required for MPK4 activation and regulates tissue-specific and temperature-dependent cell death in Arabidopsis.
    J Biol Chem. 2006 Dec 1;281(48):36969-76 PMID: 17023433
Article Info
Journal
Planta
Abbr.
Planta
ISSN
0032-0935
DOI
10.1007/s00425-008-0705-5
Published
2008-05-00
Epub
2008-00-19
Pages
1333-42
Language
English
Region
Germany
NLM ID
1250576
Subset
IM
External Links
PubMed source DOI: 10.1007/s00425-008-0705-5 View journal details
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