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PMID: 1828339 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Footprint of the sigma protein: a re-examination.

Biochemical and biophysical research communications ·Vol. 177 ·No. 1 ·1991-05-31 ·Pages 140-4

Wellman A, Meares CF

Abstract

Escherichia coli RNA Polymerase is a multi-subunit enzyme that catalyzes RNA synthesis, using DNA as a template. The sigma subunit of this enzyme plays an important role in the recognition of promoter sites on DNA. Using DNase I footprinting, Utpala Ramesh and Claude F. Meares [(1989) Biochem. Biophys. Res. Comm. 160, 121-125] reported that in the absence of the other subunits, sigma binds specifically to the bacteriophage lambda PR promoter DNA sequence. We are unable to reproduce that result.

MeSH Terms

Bacteriophage lambda/genetics DNA-Directed RNA Polymerases/isolation & purification,metabolism Deoxyribonuclease I Escherichia coli/enzymology Macromolecular Substances Nucleotide Mapping Promoter Regions, Genetic Protein Binding Sigma Factor/isolation & purification,metabolism Templates, Genetic

Chemicals

Macromolecular Substances Sigma Factor DNA-Directed RNA Polymerases Deoxyribonuclease I

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Wellman A

Chemistry Department, University of California, Davis 95616.

Meares C F

Article Info

Journal

Biochemical and biophysical research communications

Abbr.

Biochem Biophys Res Commun

ISSN

0006-291X

DOI

10.1016/0006-291x(91)91959-g

Published

1991-05-31

Pages

140-4

Language

English

Region

United States

NLM ID

0372516

Subset

Grants

NIGMS NIH HHS · GM25909 · United States

External Links

PubMed source DOI: 10.1016/0006-291x(91)91959-g View journal details

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Footprint of the sigma protein: a re-examination.

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