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PMID: 18261457 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Interaction with dopamine D2 receptor enhances expression of transient receptor potential channel 1 at the cell surface.

Biochimica et biophysica acta ·Vol. 1778 ·No. 4 ·2008-04-00 ·Pages 974-82

Hannan MA, Kabbani N, Paspalas CD, Levenson R

Abstract

Receptor signaling is mediated by direct protein interaction with various types of cytoskeletal, adapter, effector, and additional receptor molecules. In brain tissue and in cultured neurons, activation of dopamine D2 receptors (D2Rs) has been found to impact cellular calcium signaling. Using a yeast two-hybrid approach, we have uncovered a direct physical interaction between the D2R and the transient receptor potential channel (TRPC) subtypes 1, 4 and 5. The TRPC/D2R interaction was further validated by GST-pulldown assays and coimmunoprecipitation from mammalian brain. Ultrastructural analysis of TRPC1 and D2R expression indicates colocalization of the two proteins within the cell body and dendrites of cortical neurons. In cultured cells, expression of D2Rs was found to increase expression of TRPC1 at the cell surface by 50%. These findings shed new light on the constituents of the D2R signalplex, and support the involvement of D2Rs in cellular calcium signaling pathways via a novel link to TRPC channels.

MeSH Terms
Amino Acid Sequence Animals Binding Sites Biotinylation Cell Line Cerebral Cortex/cytology,metabolism,ultrastructure Humans Molecular Sequence Data Neurons/cytology,metabolism,ultrastructure Protein Binding Protein Interaction Mapping Protein Structure, Tertiary Protein Transport Rats Receptors, Dopamine D2/chemistry,metabolism Synapses/metabolism,ultrastructure TRPC Cation Channels/chemistry,metabolism
Chemicals
Receptors, Dopamine D2 TRPC Cation Channels transient receptor potential cation channel, subfamily C, member 1
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hannan Meredith A
Department of Pharmacology, Penn State University College of Medicine, 500 University Drive, Hershey, PA 17033, USA.
Kabbani Nadine
Paspalas Constantinos D
Levenson Robert
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Article Info
Journal
Biochimica et biophysica acta
Abbr.
Biochim Biophys Acta
ISSN
0006-3002
DOI
10.1016/j.bbamem.2008.01.011
Published
2008-04-00
Epub
2008-00-18
Pages
974-82
Language
English
Region
Netherlands
NLM ID
0217513
PMCID
PMC2312457
Subset
IM
Grants
NIMH NIH HHS · P50 MH068789 · United States
NIMH NIH HHS · P50 MH068789-020001 · United States
NIMH NIH HHS · MH 068789 · United States
External Links
PubMed source DOI: 10.1016/j.bbamem.2008.01.011 PMC Full Text
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