Selenoprotein A component of the glycine reductase complex from Clostridium purinolyticum: nucleotide sequence of the gene shows that selenocysteine is encoded by UGA.

Journal of bacteriology ·Vol. 173 ·No. 6 ·1991-03-00 ·Pages 2093-8

Garcia GE, Stadtman TC

Abstract

The gene encoding the selenoprotein A component of glycine reductase was isolated from Clostridium purinolyticum. The nucleotide sequence of this gene (grdA) was determined. The opal termination codon (TGA) was found in-frame at the position corresponding to the location of the selenocysteine residue in the gene product. A comparison of the nucleotide sequences and secondary mRNA structures corresponding to the selenoprotein A gene and the fdhF gene of Escherichia coli formate dehydrogenase shows that there is a similar potential for regulation of the specific insertion of selenocysteine at the UGA codon.

Related Genes

grdA

MeSH Terms

Amino Acid Oxidoreductases/genetics Amino Acid Sequence Bacterial Proteins/genetics Base Sequence Cloning, Molecular Clostridium/genetics Codon Cysteine/analogs & derivatives,genetics Genes, Bacterial Molecular Sequence Data Molecular Structure Multienzyme Complexes/genetics Nucleic Acid Conformation RNA, Messenger/ultrastructure Restriction Mapping Selenium Selenocysteine Selenoproteins

Chemicals

Bacterial Proteins Codon Multienzyme Complexes RNA, Messenger Selenoproteins selenoprotein A Selenocysteine Amino Acid Oxidoreductases glycine reductase Selenium Cysteine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Garcia G E

Laboratory of Biochemistry, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892.

Stadtman T C

References (22)

22 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.173.6.2093-2098.1991

Published

1991-03-00

Pages

2093-8

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC207745

Subset

Databases

GENBANK

M55254

Corrections

ErratumIn

PMID: 1830304

External Links

PubMed source DOI: 10.1128/jb.173.6.2093-2098.1991 PMC Full Text View journal details

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Selenoprotein A component of the glycine reductase complex from Clostridium purinolyticum: nucleotide sequence of the gene shows that selenocysteine is encoded by UGA.

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