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PMID: 18094954 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Candidate genes and QTLs for fruit ripening and softening in melon.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik ·Vol. 116 ·No. 4 ·2008-02-00 ·Pages 589-602

Moreno E, Obando JM, Dos-Santos N, Fernández-Trujillo JP, Monforte AJ, Garcia-Mas J

Abstract

Different factors affect the quality of melon fruit and among them long shelf life is critical from the consumer's point of view. In melon, cultivars showing both climacteric and non-climacteric ripening types are found. In this study we have investigated climacteric ripening and fruit softening using a collection of near-isogenic lines (NILs) derived from the non-climacteric melon parental lines PI 161375 (SC) and "Piel de Sapo" (PS). Surprisingly, we found that QTL eth3.5 in NIL SC3-5b induced a climacteric-ripening phenotype with increased respiration and ethylene levels. Data suggest that the non-climacteric phenotypes from PI 161375 and "Piel de Sapo" may be the result of mutations in different genes. Several QTLs for fruit flesh firmness were also detected. Candidate genes putatively involved in ethylene regulation, biosynthesis and perception and cell wall degradation were mapped and some colocations with QTLs were observed. These results may provide additional data towards understanding of non-climacteric ripening in melon.

MeSH Terms
Cell Respiration Cell Wall/metabolism Chromosome Mapping Cucurbitaceae/genetics,growth & development DNA, Plant Ethylenes/metabolism Fruit/chemistry,genetics,growth & development Gene Expression Regulation, Plant Genes, Plant/genetics Phenotype Pigmentation/genetics,physiology Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Polymorphism, Single Nucleotide/genetics Quantitative Trait Loci
Chemicals
DNA, Plant Ethylenes ethylene
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Moreno Eduard
IRTA, Centre de Recerca en Agrigenòmica CSIC-IRTA-UAB, Carretera de Cabrils Km2, 08348, Cabrils, Barcelona, Spain.
Obando Javier M
Dos-Santos Noelia
Fernández-Trujillo J Pablo
Monforte Antonio J
Garcia-Mas Jordi
References (32)
32 references, click to expand
  1. Identification and characterisation of a melon genomic region containing a resistance gene cluster from a constructed BAC library. Microcolinearity between Cucumis melo and Arabidopsis thaliana.
    Plant Mol Biol. 2003 Mar;51(5):703-18 PMID: 12678558
  2. Polygalacturonase gene expression in ripe melon fruit supports a role for polygalacturonase in ripening-associated pectin disassembly.
    Plant Physiol. 1998 Jun;117(2):363-73 PMID: 9625689
  3. Single-nucleotide polymorphisms detected in expressed sequence tags of melon (Cucumis melo L.).
    Genome. 2004 Apr;47(2):352-60 PMID: 15060588
  4. Ethylene regulation of fruit softening and cell wall disassembly in Charentais melon.
    J Exp Bot. 2007;58(6):1281-90 PMID: 17308329
  5. Stage- and tissue-specific expression of ethylene receptor homolog genes during fruit development in muskmelon.
    Plant Physiol. 1999 May;120(1):321-30 PMID: 10318709
  6. Identification of quantitative trait loci involved in fruit quality traits in melon (Cucumis melo L.).
    Theor Appl Genet. 2004 Feb;108(4):750-8 PMID: 14576985
  7. Biochemical diversity among the 1-amino-cyclopropane-1-carboxylate synthase isozymes encoded by the Arabidopsis gene family.
    J Biol Chem. 2003 Dec 5;278(49):49102-12 PMID: 12968022
  8. Ripening in the tomato Green-ripe mutant is inhibited by ectopic expression of a protein that disrupts ethylene signaling.
    Proc Natl Acad Sci U S A. 2006 May 16;103(20):7923-8 PMID: 16682641
  9. The ethylene-regulated expression of CS-ETR2 and CS-ERS genes in cucumber plants and their possible involvement with sex expression in flowers.
    Plant Cell Physiol. 2000 May;41(5):608-16 PMID: 10929944
  10. Molecular and genetic characterization of a non-climacteric phenotype in melon reveals two loci conferring altered ethylene response in fruit.
    Plant Physiol. 2002 May;129(1):300-9 PMID: 12011360
  11. Basic local alignment search tool.
    J Mol Biol. 1990 Oct 5;215(3):403-10 PMID: 2231712
  12. Use of genomics tools to isolate key ripening genes and analyse fruit maturation in tomato.
    J Exp Bot. 2002 Oct;53(377):2023-30 PMID: 12324526
  13. Mapping with a few plants: using selective mapping for microsatellite saturation of the Prunus reference map.
    Genetics. 2005 Nov;171(3):1305-9 PMID: 16118196
  14. Primer3 on the WWW for general users and for biologist programmers.
    Methods Mol Biol. 2000;132:365-86 PMID: 10547847
  15. Mapping quantitative physiological traits in apple (Malus x domestica Borkh.).
    Plant Mol Biol. 2003 Jun;52(3):511-26 PMID: 12956523
  16. A MADS-box gene necessary for fruit ripening at the tomato ripening-inhibitor (rin) locus.
    Science. 2002 Apr 12;296(5566):343-6 PMID: 11951045
  17. Simple-sequence repeat markers used in merging linkage maps of melon (Cucumis melo L.).
    Theor Appl Genet. 2005 Mar;110(5):802-11 PMID: 15700148
  18. Cloning of an auxin-responsive 1-aminocyclopropane-1-carboxylate synthase gene (CMe-ACS2) from melon and the expression of ACS genes in etiolated melon seedlings and melon fruits.
    Plant Sci. 2000 Nov 6;159(2):173-181 PMID: 11074269
  19. Structure and expression of three genes encoding ACC oxidase homologs from melon (Cucumis melo L.).
    Mol Gen Genet. 1996 Apr 24;251(1):81-90 PMID: 8628251
  20. Nucleotide sequence of a cDNA for 1-aminocyclopropane-1-carboxylate synthase from melon fruits.
    Plant Physiol. 1995 Jan;107(1):297-8 PMID: 7870831
  21. QTL analysis of fruit quality in fresh market tomato: a few chromosome regions control the variation of sensory and instrumental traits.
    J Exp Bot. 2002 Oct;53(377):2089-98 PMID: 12324532
  22. Nuclear events in ethylene signaling: a transcriptional cascade mediated by ETHYLENE-INSENSITIVE3 and ETHYLENE-RESPONSE-FACTOR1.
    Genes Dev. 1998 Dec 1;12(23):3703-14 PMID: 9851977
  23. Physiological relationships among physical, sensory, and morphological attributes of texture in tomato fruits.
    J Exp Bot. 2007;58(8):1915-25 PMID: 17452757
  24. Development of a genomic library of near isogenic lines (NILs) in melon (Cucumis melo L.) from the exotic accession PI161375.
    Theor Appl Genet. 2005 Dec;112(1):139-48 PMID: 16208502
  25. Fruit ripening mutants yield insights into ripening control.
    Curr Opin Plant Biol. 2007 Jun;10(3):283-9 PMID: 17442612
  26. Construction of a reference linkage map for melon.
    Genome. 2001 Oct;44(5):836-45 PMID: 11681608
  27. Expression of ACC oxidase antisense gene inhibits ripening of cantaloupe melon fruits.
    Nat Biotechnol. 1996 Jul;14(7):862-6 PMID: 9631011
  28. An introgression line population of Lycopersicon pennellii in the cultivated tomato enables the identification and fine mapping of yield-associated QTL.
    Genetics. 1995 Nov;141(3):1147-62 PMID: 8582620
  29. Cell wall metabolism in fruit softening and quality and its manipulation in transgenic plants.
    Plant Mol Biol. 2001 Sep;47(1-2):311-40 PMID: 11554479
  30. Cell turgor changes associated with ripening in tomato pericarp tissue.
    Plant Physiol. 1991 Oct;97(2):814-6 PMID: 16668472
  31. MELOGEN: an EST database for melon functional genomics.
    BMC Genomics. 2007 Sep 03;8:306 PMID: 17767721
  32. A naturally occurring epigenetic mutation in a gene encoding an SBP-box transcription factor inhibits tomato fruit ripening.
    Nat Genet. 2006 Aug;38(8):948-52 PMID: 16832354
Article Info
Journal
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
Abbr.
Theor Appl Genet
ISSN
0040-5752
DOI
10.1007/s00122-007-0694-y
Published
2008-02-00
Epub
2007-00-20
Pages
589-602
Language
English
Region
Germany
NLM ID
0145600
Subset
IM
External Links
PubMed source DOI: 10.1007/s00122-007-0694-y View journal details
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