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PMID: 1809397 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Distinct determinants on collagen support alpha 2 beta 1 integrin-mediated platelet adhesion and platelet activation.

Cell regulation ·Vol. 2 ·No. 11 ·1991-11-00 ·Pages 905-13

Santoro SA, Walsh JJ, Staatz WD, Baranski KJ

Abstract

Recent studies have revealed that the sequence of amino acids asp-gly-glu-ala represents an essential determinant of the site within the alpha 1(I)-CB3 fragment of collagen recognized by the alpha 2 beta 1 integrin cell surface collagen receptor (Staatz et al., 1991). Studies employing chemical modifications of collagen amino acid side chains confirm both the essential nature of the acidic side chains of aspartic acid and glutamic acid residues and the nonessentiality of lysine epsilon-amino groups in supporting adhesion mediated by the alpha 2 beta 1 integrin. The approach also indicates the presence of a distinct determinant on collagen separate from the alpha 2 beta 1 recognition site that contains essential lysine side chains and that is necessary for subsequent interactions with the platelet surface that give rise to collagen-induced platelet activation and secretion. The two-step, two-site model for cellular signaling involving both an integrin and a signal-transducing coreceptor suggested by these data may be common to other integrin-mediated processes.

MeSH Terms
Acetylation Amino Acid Sequence Aspartic Acid/chemistry Binding Sites Blood Platelets/cytology Collagen/chemistry,metabolism Glutamates/chemistry Humans In Vitro Techniques Integrins/physiology Kinetics Lysine/chemistry Molecular Sequence Data Platelet Activation Platelet Adhesiveness Structure-Activity Relationship
Chemicals
Glutamates Integrins Aspartic Acid Collagen Lysine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Santoro S A
Department of Pathology, Washington University School of Medicine, St. Louis, Missouri 63110.
Walsh J J
Staatz W D
Baranski K J
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Article Info
Journal
Cell regulation
Abbr.
Cell Regul
ISSN
1044-2030
DOI
10.1091/mbc.2.11.905
Published
1991-11-00
Pages
905-13
Language
English
Region
United States
NLM ID
9005331
PMCID
PMC361889
Subset
IM
Grants
NHLBI NIH HHS · HL-40506 · United States
External Links
PubMed source DOI: 10.1091/mbc.2.11.905 PMC Full Text
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