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PMID: 18085823 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Hemophagocytic macrophages harbor Salmonella enterica during persistent infection.

PLoS pathogens ·Vol. 3 ·No. 12 ·2007-12-00 ·Pages e193

Nix RN, Altschuler SE, Henson PM, Detweiler CS

Abstract

Salmonella enterica subspecies can establish persistent, systemic infections in mammals, including human typhoid fever. Persistent S. enterica disease is characterized by an initial acute infection that develops into an asymptomatic chronic infection. During both the acute and persistent stages, the bacteria generally reside within professional phagocytes, usually macrophages. It is unclear how salmonellae can survive within macrophages, cells that evolved, in part, to destroy pathogens. Evidence is presented that during the establishment of persistent murine infection, macrophages that contain S. enterica serotype Typhimurium are hemophagocytic. Hemophagocytic macrophages are characterized by the ingestion of non-apoptotic cells of the hematopoietic lineage and are a clinical marker of typhoid fever as well as certain other infectious and genetic diseases. Cell culture assays were developed to evaluate bacterial survival in hemophagocytic macrophages. S. Typhimurium preferentially replicated in macrophages that pre-phagocytosed viable cells, but the bacteria were killed in macrophages that pre-phagocytosed beads or dead cells. These data suggest that during persistent infection hemophagocytic macrophages may provide S. Typhimurium with a survival niche.

MeSH Terms
Animals Bone Marrow Cells/cytology,drug effects,immunology Disease Models, Animal Female Host-Pathogen Interactions Humans Jurkat Cells Lipopolysaccharides/pharmacology Liver/microbiology,pathology Macrophages/microbiology,pathology,physiology Mice Mice, Inbred Strains Phagocytosis/physiology Salmonella Infections/blood,microbiology Salmonella enterica/pathogenicity,physiology Spleen/microbiology,pathology
Chemicals
Lipopolysaccharides
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Nix Rebecca N
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado, United States of America.
Altschuler Sarah E
Henson Peter M
Detweiler Corrella S
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Article Info
Journal
PLoS pathogens
Abbr.
PLoS Pathog
ISSN
1553-7374
DOI
10.1371/journal.ppat.0030193
Published
2007-12-00
Pages
e193
Language
English
Region
United States
NLM ID
101238921
PMCID
PMC2134957
Subset
IM
Grants
NIAID NIH HHS · R56 AI063116 · United States
NIAID NIH HHS · R01 AI072492 · United States
NIAID NIH HHS · AI063116 · United States
NIAID NIH HHS · R56 AI063116-01A1 · United States
NIAID NIH HHS · R01 AI072492-01A1 · United States
NIGMS NIH HHS · R01 GM061031 · United States
NIAID NIH HHS · AI072492 · United States
NIGMS NIH HHS · GM61031 · United States
External Links
PubMed source DOI: 10.1371/journal.ppat.0030193 PMC Full Text View journal details
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