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PMID: 18083823 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The acyl-homoserine lactone-type quorum-sensing system modulates cell motility and virulence of Erwinia chrysanthemi pv. zeae.

Journal of bacteriology ·Vol. 190 ·No. 3 ·2008-02-00 ·Pages 1045-53

Hussain MB, Zhang HB, Xu JL, Liu Q, Jiang Z, Zhang LH

Abstract

Erwinia chrysanthemi pv. zeae is one of the Erwinia chrysanthemi pathovars that infects on both dicotyledons and monocotyledons. However, little is known about the molecular basis and regulatory mechanisms of its virulence. By using a transposon mutagenesis approach, we cloned the genes coding for an E. chrysanthemi pv. zeae synthase of acyl-homoserine lactone (AHL) quorum-sensing signals (expI(Ecz)) and a cognate response regulator (expR(Ecz)). Chromatography analysis showed that expI(Ecz) encoded production of the AHL signal N-(3-oxo-hexanoyl)-homoserine lactone (OHHL). Null mutation of expI(Ecz) in the E. chrysanthemi pv. zeae strain EC1 abolished AHL production, increased bacterial swimming and swarming motility, disabled formation of multicell aggregates, and attenuated virulence of the pathogen on potato tubers. The mutation also marginally reduced the inhibitory activity of E. chrysanthemi pv. zeae on rice seed germination. The mutant phenotypes were rescued by either exogenous addition of AHL signal or in trans expression of expI(Ecz). These data demonstrate that the AHL-type QS signal plays an essential role in modulation of E. chrysanthemi pv. zeae cell motility and the ability to form multicell aggregates and is involved in regulation of bacterial virulence.

MeSH Terms
Acyl-Butyrolactones/metabolism Amino Acid Sequence Bacterial Adhesion Bacterial Proteins/genetics,metabolism Base Sequence Cell Movement Dickeya chrysanthemi/genetics,metabolism,pathogenicity,physiology Gene Expression Regulation, Bacterial Molecular Sequence Data Oryza/microbiology Quorum Sensing Seeds/microbiology Sequence Analysis, DNA Solanum tuberosum/microbiology Trans-Activators/genetics,metabolism Virulence
Chemicals
Acyl-Butyrolactones Bacterial Proteins ExpR protein, Erwinia Trans-Activators expI protein, Erwinia
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Hussain Mumtaz B B M
Institute of Molecular and Cell Biology, 61 Biopolis Drive, Singapore 138673.
Zhang Hai-Bao
Xu Jin-Ling
Liu Qiongguang
Jiang Zide
Zhang Lian-Hui
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
1098-5530
DOI
10.1128/JB.01472-07
Published
2008-02-00
Epub
2007-00-14
Pages
1045-53
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC2223575
Subset
IM
Databases
GENBANK
EU142019
External Links
PubMed source DOI: 10.1128/JB.01472-07 PMC Full Text View journal details
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