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PMID: 18080174 Published · ppublish English Comparative Study Journal Article

Mink growth hormone structural-functional relationships: effects of renaturing and storage conditions.

The protein journal ·Vol. 27 ·No. 3 ·2008-04-00 ·Pages 170-80

Borromeo V, Sereikaite J, Bumelis VA, Secchi C, Scirè A, Ausili A, D'Auria S, Tanfani F

Abstract

Fourier-transform infrared spectroscopy, in vitro bioassay and enzyme-linked immunoassay were used to study the structural-functional relationships of recombinant mink growth hormone (mGH), refolded and stored under different conditions. Porcine GH (pGH) was synthesized and used as an example. These two hormones, when refolded and stored the same way, had the same secondary structures, biological and immunological efficacy, and biological potency. Only the immunological potency differed, mGH being significantly less potent than pGH. Renaturation pH and storing frozen or at 4 degrees C in 5% glycerol did not affect either the secondary structure or the activity. However, freeze-drying raised the content of buried alpha-helices and lowered that of solvated alpha-helices and of unordered structures. These conformational changes were associated with a reduction of immunological and biological potency of mGH and of immunological potency of pGH. These findings provide original information on the secondary structure of mGH, and show that conformational changes induced by lyophilization adversely affect its activity.

MeSH Terms
Amino Acid Sequence Animals Cell Line, Tumor Freeze Drying Growth Hormone/chemistry,genetics,immunology Mice Mink/genetics,immunology Molecular Sequence Data Protein Renaturation Protein Structure, Secondary Recombinant Proteins/chemistry,genetics,immunology Sequence Alignment Spectroscopy, Fourier Transform Infrared Structure-Activity Relationship Swine Temperature
Chemicals
Recombinant Proteins Growth Hormone
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Borromeo Vitaliano
Department of Animal Pathology and Health, Faculty of Veterinary Medicine, University of Milan, Milan, Italy. vitaliano.borromeo@unimi.it
Sereikaite Jolanta
Bumelis Vladas-Algirdas
Secchi Camillo
Scirè Andrea
Ausili Alessio
D'Auria Sabato
Tanfani Fabio
References (58)
58 references, click to expand
  1. Recombinant porcine somatotropin and dietary protein enhance protein synthesis in growing pigs.
    J Nutr. 1993 Mar;123(3):529-40 PMID: 7681874
  2. The hydration of amides in helices; a comprehensive picture from molecular dynamics, IR, and NMR.
    Protein Sci. 2003 Mar;12(3):520-31 PMID: 12592022
  3. Endocrinologic adaptations to wintertime fasting in the male American mink (Mustela vison).
    Exp Biol Med (Maywood). 2005 Oct;230(9):612-20 PMID: 16179729
  4. Improved tools for biological sequence comparison.
    Proc Natl Acad Sci U S A. 1988 Apr;85(8):2444-8 PMID: 3162770
  5. Anabolic action of growth hormone.
    Ann N Y Acad Sci. 1968 Feb 5;148(2):408-18 PMID: 4871875
  6. Infrared amide I' band of the coiled coil.
    Biochemistry. 1996 Feb 6;35(5):1383-6 PMID: 8634267
  7. The infrared absorption of amino acid side chains.
    Prog Biophys Mol Biol. 2000;74(3-5):141-73 PMID: 11226511
  8. Infrared absorbances of protein side chains.
    Anal Biochem. 1998 Mar 1;257(1):1-11 PMID: 9512765
  9. Temperature-, SDS-, and pH-induced conformational changes in protein disulfide oxidoreductase from the archaeon Pyrococcus furiosus: a dynamic simulation and fourier transform infrared spectroscopic study.
    J Proteome Res. 2005 Nov-Dec;4(6):1972-80 PMID: 16335941
  10. An algorithm for secondary structure determination in proteins based on sequence similarity.
    FEBS Lett. 1986 Sep 15;205(2):303-8 PMID: 3743779
  11. Effects of complexation with in vivo enhancing monoclonal antibodies on activity of growth hormone in two responsive cell culture systems.
    J Mol Endocrinol. 1999 Dec;23(3):307-13 PMID: 10601976
  12. Human growth hormone and extracellular domain of its receptor: crystal structure of the complex.
    Science. 1992 Jan 17;255(5042):306-12 PMID: 1549776
  13. Examination of the secondary structure of proteins by deconvolved FTIR spectra.
    Biopolymers. 1986 Mar;25(3):469-87 PMID: 3697478
  14. D-trehalose/D-maltose-binding protein from the hyperthermophilic archaeon Thermococcus litoralis: the binding of trehalose and maltose results in different protein conformational states.
    Proteins. 2006 Jun 1;63(4):754-67 PMID: 16532450
  15. New insight into protein secondary structure from resolution-enhanced infrared spectra.
    Biochim Biophys Acta. 1988 Jan 29;952(2):115-30 PMID: 3276352
  16. The conformation-sensitive immunoassay: a membrane based ELISA system for identifying antibodies sensitive to alterations of protein conformation.
    Mol Immunol. 1990 Jun;27(6):495-502 PMID: 2166235
  17. In-vitro secretion of prolactin and growth hormone in the presence of melatonin by pituitary gland from mink kept under long or short days.
    J Endocrinol. 1988 Nov;119(2):287-92 PMID: 3199061
  18. Reduced beta-strand content in apoprotein B-100 in smaller and denser low-density lipoprotein subclasses as probed by Fourier-transform infrared spectroscopy.
    Biochem J. 1997 Mar 15;322 ( Pt 3):765-9 PMID: 9148747
  19. Somatotropin regulation of protein metabolism in pigs.
    J Anim Sci. 2004;82 E-Suppl:E207-213 PMID: 15471799
  20. The biology of somatotropin in adipose tissue growth and nutrient partitioning.
    J Nutr. 2000 Nov;130(11):2623-5 PMID: 11053496
  21. SWISS-MODEL and the Swiss-PdbViewer: an environment for comparative protein modeling.
    Electrophoresis. 1997 Dec;18(15):2714-23 PMID: 9504803
  22. Monoclonal antibodies recognize conformational alterations leading to decreased bioactivity of human growth hormone.
    Mol Cell Endocrinol. 1984 Nov;38(1):75-80 PMID: 6083888
  23. What vibrations tell us about proteins.
    Q Rev Biophys. 2002 Nov;35(4):369-430 PMID: 12621861
  24. Effects of exogenous porcine somatotropin on the carcass composition, hormonal and metabolic profiles, lipogenic capacity, and binding of insulin to erythrocyte receptors of fast- versus slow-growing swine.
    J Anim Sci. 1993 Jul;71(7):1786-95 PMID: 8349505
  25. A heuristic approach to predicting the tertiary structure of bovine somatotropin.
    Biochemistry. 1991 May 7;30(18):4389-98 PMID: 2021631
  26. Sensitivity of hybrid ovine/rat GH receptors to oGH and rat GH in transfected FDC-P1 mouse myeloid cells in vitro.
    Mol Cell Biochem. 2002 Sep;238(1-2):137-43 PMID: 12349901
  27. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays.
    J Immunol Methods. 1983 Dec 16;65(1-2):55-63 PMID: 6606682
  28. The X-ray structure of a growth hormone-prolactin receptor complex.
    Nature. 1994 Dec 1;372(6505):478-81 PMID: 7984244
  29. Porter: a new, accurate server for protein secondary structure prediction.
    Bioinformatics. 2005 Apr 15;21(8):1719-20 PMID: 15585524
  30. The secondary structure of pressure- and temperature-induced aggregates of equine serum albumin studied by FT-IR spectroscopy.
    Biochim Biophys Acta. 2006 Aug;1764(8):1407-12 PMID: 16920040
  31. Cloning and sequence analysis of mink growth hormone cDNA.
    Biochem Biophys Res Commun. 1990 Dec 31;173(3):1200-4 PMID: 2268323
  32. The effects of formulation variables on the stability of freeze-dried human growth hormone.
    Pharm Res. 1991 Apr;8(4):427-36 PMID: 1871037
  33. Quantitative studies of the structure of proteins in solution by Fourier-transform infrared spectroscopy.
    Prog Biophys Mol Biol. 1993;59(1):23-56 PMID: 8419985
  34. Surface-core relationships in human low density lipoprotein as studied by infrared spectroscopy.
    J Biol Chem. 1995 Apr 21;270(16):9192-6 PMID: 7721835
  35. Temperature-induced molten globule-like state in human alpha1-acid glycoprotein: an infrared spectroscopic study.
    Biochemistry. 2005 Dec 13;44(49):15997-6006 PMID: 16331959
  36. Identification and characterization of an anti-isoaspartic acid monoclonal antibody.
    J Protein Chem. 1992 Dec;11(6):657-63 PMID: 1281635
  37. Amino acid modifications in canine, equine and porcine pituitary growth hormones, identified by peptide-mass mapping.
    J Chromatogr B Biomed Sci Appl. 2001 Jun 15;757(2):237-45 PMID: 11417868
  38. Different colony-stimulating factors are detected by the "interleukin-3"-dependent cell lines FDC-Pl and 32D cl-23.
    Blood. 1984 Oct;64(4):786-90 PMID: 6434004
  39. SWISS-MODEL: An automated protein homology-modeling server.
    Nucleic Acids Res. 2003 Jul 1;31(13):3381-5 PMID: 12824332
  40. Estimation of amino acid residue side-chain absorption in the infrared spectra of protein solutions in heavy water.
    Biopolymers. 1975 Apr;14(4):679-94 PMID: 1156632
  41. Crystal structure of an antagonist mutant of human growth hormone, G120R, in complex with its receptor at 2.9 A resolution.
    J Biol Chem. 1996 Dec 13;271(50):32197-203 PMID: 8943276
  42. Three-dimensional structure of a genetically engineered variant of porcine growth hormone.
    Proc Natl Acad Sci U S A. 1987 Sep;84(18):6434-7 PMID: 2819877
  43. Binding of glutamine to glutamine-binding protein from Escherichia coli induces changes in protein structure and increases protein stability.
    Proteins. 2005 Jan 1;58(1):80-7 PMID: 15517590
  44. Improving the accuracy of protein secondary structure prediction using structural alignment.
    BMC Bioinformatics. 2006 Jun 14;7:301 PMID: 16774686
  45. SOPMA: significant improvements in protein secondary structure prediction by consensus prediction from multiple alignments.
    Comput Appl Biosci. 1995 Dec;11(6):681-4 PMID: 8808585
  46. Vibrational spectroscopy and conformation of peptides, polypeptides, and proteins.
    Adv Protein Chem. 1986;38:181-364 PMID: 3541539
  47. Efficient bacterial expression of bovine and porcine growth hormones.
    DNA. 1983;2(1):37-45 PMID: 6303731
  48. Comparison of a structural and a functional epitope.
    J Mol Biol. 1993 Dec 5;234(3):554-63 PMID: 7504735
  49. Isolation and characterization of porcine somatotropin containing a succinimide residue in place of aspartate129.
    Protein Sci. 1992 Dec;1(12):1634-41 PMID: 1363933
  50. Monoclonal antibodies as a probe for the unfolding of porcine growth hormone.
    J Immunol Methods. 2003 Jan 15;272(1-2):107-15 PMID: 12505716
  51. Cascaded multiple classifiers for secondary structure prediction.
    Protein Sci. 2000 Jun;9(6):1162-76 PMID: 10892809
  52. Effects of frequency of treatment with recombinant equine somatotropin on selected biological responses in geldings.
    Domest Anim Endocrinol. 2002 May;22(3):127-43 PMID: 11934522
  53. Growth hormone heterogeneity: genes, isohormones, variants, and binding proteins.
    Endocr Rev. 1991 Nov;12(4):424-49 PMID: 1760996
  54. Growth hormone.
    Lancet. 1970 May 2;1(7653):933-9 PMID: 4191561
  55. Production of recombinant mink growth hormone in E. coli.
    Appl Microbiol Biotechnol. 2007 Feb;74(2):316-23 PMID: 17103160
  56. Epidermal homeostasis: the role of the growth hormone and insulin-like growth factor systems.
    Endocr Rev. 2003 Dec;24(6):737-64 PMID: 14671001
  57. Growth hormone and prolactin--molecular and functional evolution.
    J Mammary Gland Biol Neoplasia. 2002 Jul;7(3):291-312 PMID: 12751893
  58. Technologies for the control of fat and lean deposition in livestock.
    Vet J. 2004 May;167(3):242-57 PMID: 15080873
Article Info
Journal
The protein journal
Abbr.
Protein J
ISSN
1572-3887
DOI
10.1007/s10930-007-9120-1
Email
vitaliano.borromeo@unimi.it
Published
2008-04-00
Pages
170-80
Language
English
Region
Netherlands
NLM ID
101212092
Subset
IM
External Links
PubMed source DOI: 10.1007/s10930-007-9120-1 View journal details
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