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PMID: 18070072 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Diversity of nifH gene pools in the rhizosphere of two cultivars of sorghum (Sorghum bicolor) treated with contrasting levels of nitrogen fertilizer.

FEMS microbiology letters ·Vol. 279 ·No. 1 ·2008-02-00 ·Pages 15-22

Coelho MR, de Vos M, Carneiro NP, Marriel IE, Paiva E, Seldin L

Abstract

The diversity of nitrogen-fixing bacteria was assessed in the rhizospheres of two cultivars of sorghum (IS 5322-C and IPA 1011) sown in Cerrado soil amended with two levels of nitrogen fertilizer (12 and 120 kg ha(-1)). The nifH gene was amplified directly from DNA extracted from the rhizospheres, and the PCR products cloned and sequenced. Four clone libraries were generated from the nifH fragments and 245 sequences were obtained. Most of the clones (57%) were closely related to nifH genes of uncultured bacteria. NifH clones affiliated with Azohydromonas spp., Ideonella sp., Rhizobium etli and Bradyrhizobium sp. were found in all libraries. Sequences affiliated with Delftia tsuruhatensis were found in the rhizosphere of both cultivars sown with high levels of nitrogen, while clones affiliated with Methylocystis sp. were detected only in plants sown under low levels of nitrogen. Moreover, clones affiliated with Paenibacillus durus could be found in libraries from the cultivar IS 5322-C sown either in high or low amounts of fertilizer. This study showed that the amount of nitrogen used for fertilization is the overriding determinative factor that influenced the nitrogen-fixing community structures in sorghum rhizospheres cultivated in Cerrado soil.

MeSH Terms

Bacteria/classification,genetics Biodiversity DNA, Bacterial/chemistry,genetics,isolation & purification Fertilizers Molecular Sequence Data Oxidoreductases/genetics Sequence Analysis, DNA Sequence Homology Soil Microbiology Sorghum/microbiology

Chemicals

DNA, Bacterial Fertilizers Oxidoreductases nitrogenase reductase

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Coelho Marcia Reed Rodrigues

Instituto de Microbiologia Prof. Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.

de Vos Marjon

Carneiro Newton Portilho

Marriel Ivanildo Evódio

Paiva Edilson

Seldin Lucy

Article Info

Journal

FEMS microbiology letters

Abbr.

FEMS Microbiol Lett

ISSN

0378-1097

DOI

10.1111/j.1574-6968.2007.00975.x

Published

2008-02-00

Epub

2007-00-07

Pages

15-22

Language

English

Region

England

NLM ID

7705721

Subset

Databases

GENBANK

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PubMed source DOI: 10.1111/j.1574-6968.2007.00975.x View journal details

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Diversity of nifH gene pools in the rhizosphere of two cultivars of sorghum (Sorghum bicolor) treated with contrasting levels of nitrogen fertilizer.

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