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PMID: 18056638 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The interplay between the master transcription factor PU.1 and miR-424 regulates human monocyte/macrophage differentiation.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 104 ·No. 50 ·2007-12-11 ·Pages 19849-54

Rosa A, Ballarino M, Sorrentino A, Sthandier O, De Angelis FG, Marchioni M, Masella B, Guarini A, Fatica A, Peschle C, Bozzoni I

Abstract

We describe a pathway by which the master transcription factor PU.1 regulates human monocyte/macrophage differentiation. This includes miR-424 and the transcriptional factor NFI-A. We show that PU.1 and these two components are interlinked in a finely tuned temporal and regulatory circuitry: PU.1 activates the transcription of miR-424, and this up-regulation is involved in stimulating monocyte differentiation through miR-424-dependent translational repression of NFI-A. In turn, the decrease in NFI-A levels is important for the activation of differentiation-specific genes such as M-CSFr. In line with these data, both RNAi against NFI-A and ectopic expression of miR-424 in precursor cells enhance monocytic differentiation, whereas the ectopic expression of NFI-A has an opposite effect. The interplay among these three components was demonstrated in myeloid cell lines as well as in human CD34+ differentiation. These data point to the important role of miR-424 and NFI-A in controlling the monocyte/macrophage differentiation program.

MeSH Terms
Base Sequence Cell Differentiation Cells, Cultured Hematopoiesis Humans Macrophages/cytology,metabolism MicroRNAs/genetics Monocytes/cytology,metabolism NFI Transcription Factors/genetics,metabolism Protein Binding Proto-Oncogene Proteins/metabolism Trans-Activators/metabolism Up-Regulation
Chemicals
MicroRNAs NFI Transcription Factors NFIA protein, human Proto-Oncogene Proteins Trans-Activators proto-oncogene protein Spi-1
Authors & Affiliations
11 authors, click to expand affiliations / ORCID
Rosa A
Institute Pasteur Cenci-Bolognetti, Department of Genetics and Molecular Biology, Institute of Molecular Biology and Pathology, and Department of Cellular Biotechnologies and Hematology, University of Rome "La Sapienza", Rome, Italy.
Ballarino M
Sorrentino A
Sthandier O
De Angelis F G
Marchioni M
Masella B
Guarini A
Fatica A
Peschle C
Bozzoni I
References (30)
30 references, click to expand
  1. Characterization of the continuous, differentiating myeloid cell line (HL-60) from a patient with acute promyelocytic leukemia.
    Blood. 1979 Sep;54(3):713-33 PMID: 288488
  2. A minicircuitry comprised of microRNA-223 and transcription factors NFI-A and C/EBPalpha regulates human granulopoiesis.
    Cell. 2005 Dec 2;123(5):819-31 PMID: 16325577
  3. Quantitative assessment of gene targeting in vitro and in vivo by the pancreatic transcription factor, Pdx1. Importance of chromatin structure in directing promoter binding.
    J Biol Chem. 2002 Apr 12;277(15):13286-93 PMID: 11825903
  4. Multilineage gene expression precedes commitment in the hemopoietic system.
    Genes Dev. 1997 Mar 15;11(6):774-85 PMID: 9087431
  5. Effect of transcription-factor concentrations on leukemic stem cells.
    Blood. 2005 Sep 1;106(5):1519-24 PMID: 15914558
  6. A pancreatic islet-specific microRNA regulates insulin secretion.
    Nature. 2004 Nov 11;432(7014):226-30 PMID: 15538371
  7. CCAAT/enhancer binding protein alpha is a regulatory switch sufficient for induction of granulocytic development from bipotential myeloid progenitors.
    Mol Cell Biol. 1998 Jul;18(7):4301-14 PMID: 9632814
  8. miRNAs, cancer, and stem cell division.
    Cell. 2005 Jul 15;122(1):6-7 PMID: 16009126
  9. PU.1 (Spi-1) autoregulates its expression in myeloid cells.
    Oncogene. 1995 Oct 19;11(8):1549-60 PMID: 7478579
  10. MicroRNAs 17-5p-20a-106a control monocytopoiesis through AML1 targeting and M-CSF receptor upregulation.
    Nat Cell Biol. 2007 Jul;9(7):775-87 PMID: 17589498
  11. A new vector, based on the PolII promoter of the U1 snRNA gene, for the expression of siRNAs in mammalian cells.
    Mol Ther. 2004 Jul;10(1):191-9 PMID: 15272480
  12. Altered expression profiles of microRNAs during TPA-induced differentiation of HL-60 cells.
    Biochem Biophys Res Commun. 2004 Sep 17;322(2):403-10 PMID: 15325244
  13. MicroRNAs 221 and 222 inhibit normal erythropoiesis and erythroleukemic cell growth via kit receptor down-modulation.
    Proc Natl Acad Sci U S A. 2005 Dec 13;102(50):18081-6 PMID: 16330772
  14. MicroRNA expression and function in cancer.
    Trends Mol Med. 2006 Dec;12(12):580-7 PMID: 17071139
  15. Unilineage hematopoietic differentiation in bulk and single cell culture.
    Stem Cells. 1998;16 Suppl 1:51-73 PMID: 11012148
  16. Diversification of haematopoietic stem cells to specific lineages.
    Nat Rev Genet. 2000 Oct;1(1):57-64 PMID: 11262875
  17. Multilineage transcriptional priming and determination of alternate hematopoietic cell fates.
    Cell. 2006 Aug 25;126(4):755-66 PMID: 16923394
  18. The role of microRNAs in normal hematopoiesis and hematopoietic malignancies.
    Leukemia. 2006 Nov;20(11):1931-6 PMID: 16990772
  19. Requirement of transcription factor PU.1 in the development of multiple hematopoietic lineages.
    Science. 1994 Sep 9;265(5178):1573-7 PMID: 8079170
  20. Potential autoregulation of transcription factor PU.1 by an upstream regulatory element.
    Mol Cell Biol. 2005 Apr;25(7):2832-45 PMID: 15767686
  21. Transcriptional accessibility for genes of multiple tissues and hematopoietic lineages is hierarchically controlled during early hematopoiesis.
    Blood. 2003 Jan 15;101(2):383-9 PMID: 12393558
  22. Granulocyte inducer C/EBPalpha inactivates the myeloid master regulator PU.1: possible role in lineage commitment decisions.
    Blood. 2002 Jul 15;100(2):483-90 PMID: 12091339
  23. MAPPER: a search engine for the computational identification of putative transcription factor binding sites in multiple genomes.
    BMC Bioinformatics. 2005 Mar 30;6:79 PMID: 15799782
  24. 'Advanced' generation lentiviruses as efficient vectors for cardiomyocyte gene transduction in vitro and in vivo.
    Gene Ther. 2003 Apr;10(8):630-6 PMID: 12692591
  25. Disruption of differentiation in human cancer: AML shows the way.
    Nat Rev Cancer. 2003 Feb;3(2):89-101 PMID: 12563308
  26. Coordinate dual-gene transgenesis by lentiviral vectors carrying synthetic bidirectional promoters.
    Nat Biotechnol. 2005 Jan;23(1):108-16 PMID: 15619618
  27. NB4, a maturation inducible cell line with t(15;17) marker isolated from a human acute promyelocytic leukemia (M3).
    Blood. 1991 Mar 1;77(5):1080-6 PMID: 1995093
  28. MicroRNAs modulate hematopoietic lineage differentiation.
    Science. 2004 Jan 2;303(5654):83-6 PMID: 14657504
  29. PU.1 inhibits GATA-1 function and erythroid differentiation by blocking GATA-1 DNA binding.
    Blood. 2000 Oct 15;96(8):2641-8 PMID: 11023493
  30. miRNAMap: genomic maps of microRNA genes and their target genes in mammalian genomes.
    Nucleic Acids Res. 2006 Jan 1;34(Database issue):D135-9 PMID: 16381831
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
1091-6490
DOI
10.1073/pnas.0706963104
Published
2007-12-11
Epub
2007-00-03
Pages
19849-54
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC2148386
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.0706963104 PMC Full Text View journal details
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